Volume 6 Supplement 1
Five years of experience teaching pathology to dental students using the WebMicroscope
© Szymas and Lundin; licensee BioMed Central Ltd. 2011
Published: 30 March 2011
We describe development and evaluation of the user-friendly web based virtual microscopy - WebMicroscope for teaching and learning dental students basic and oral pathology. Traditional students microscopes were replaced by computer workstations.
The transition of the basic and oral pathology courses from light to virtual microscopy has been completed gradually over a five-year period. A pilot study was conducted in academic year 2005/2006 to estimate the feasibility of integrating virtual microscopy into a traditional light microscopy-based pathology course. The entire training set of glass slides was subsequently converted to virtual slides and placed on the WebMicroscope server. Giving access to fully digitized slides on the web with a browser and a viewer plug-in, the computer has become a perfect companion of the student.
The study material consists now of over 400 fully digitized slides which covering 15 entities in basic and systemic pathology and 15 entities in oral pathology. Digitized slides are linked with still macro- and microscopic images, organized with clinical information into virtual cases and supplemented with text files, syllabus, PowerPoint presentations and animations on the web, serving additionally as material for individual studies. After their examinations, the students rated the use of the software, quality of the images, the ease of handling the images, and the effective use of virtual slides during the laboratory practicals. Responses were evaluated on a standardized scale. Because of the positive opinions and support from the students, the satisfaction surveys had shown a progressive improvement over the past 5 years. The WebMicroscope as a didactic tool for laboratory practicals was rated over 8 on a 1-10 scale for basic and systemic pathology and 9/10 for oral pathology especially as various students’ suggestions were implemented. Overall, the quality of the images was rated as very good.
An overwhelming majority of our students regarded a possibility of using virtual slides at their convenience as highly desirable. Our students and faculty consider the use of the virtual microscope for the study of basic as well as oral pathology as a significant improvement over the light microscope.
There is an increasing tendency at medical universities to digitize whole microscope histopathological slides from teaching collections for web-based studies . Virtual microscope - WebMicroscope using digitized slides as Enhanced Compression Wavelet (ecw) file, format pioneered in aerial and satellite imagery, offer an alternative to the other web-based method of teaching pathology . It makes microscope laboratory studies in pathology more efficient. This expands a traditional microscopic study allowing the student to examine entire tissue sections. It is also opposed to static images of selected fields. In this way, digitized slides can be visualized at any magnification and moved in the x-y axis. This nearly perfectly emulates a traditional microscope and a glass slide . Virtual microscopy allows students to independently explore the entire histologic slide. Abandoning the use of conventional training microscopes and glass slides, we decided to rely on virtual microscopy to facilitate learning of pathology and to radically redesign laboratory practicals for teaching basic and oral pathology to dental students. This article describes 5 years of our experience with this experiment and some of the surprises we met in the evaluation by the students and staff.
Available scaling for Axioplan 2e microscope as a virtual slide generator
Resolution of digital camera
20x (1300 x 1030 pixels)
40x (1300 x 1030 pixels)
20x (2600 x 2060 pixels)
20x (3900 x 3090 pixels)
40x (2600 x 2060 pixels)
40x (3900 x 3090 pixels)
Implementation in basic and systemic pathology
In the fall semester of 2005, we carried out a formative evaluation of the WebMicroscopy laboratory practical for the third-year dental students course but only for one student group (8 students). Glass slides from previous unit of the laboratory practicals were digitized, placed on the WebMicroscope server and made available to the students on the internet. During the first laboratory practical, traditional student microscopes and glass slides were also available as a supplement. At the end of the first laboratory practical, students rated the Virtual Microscope as superior to the traditional microscope in terms of clarity of morphologic images, the ease of use, efficiency and accessibility. Considering this positive evaluation, subsequent practicals were conducted using exclusively the WebMicroscope. A set of over 100 histopathological slides was subsequently digitized and used in the 2005 fall semester (basic and systemic pathology) and 2006 spring semester (oral pathology). In this way, the virtual microscope has become the only device used by those students during laboratory practicals. Whereas this “experimental” group was taught with the WebMicroscopy in a computer lab with two students per computer during the two introductory semesters, remaining students were taught in a traditional laboratory. An assistant or adjunct was present in all sites to interact with the students at individual workstations or microscopes.
Implementation in oral pathology
In the second semester of third year course, students have 15 laboratory practicals. Students examine microscopic slides and associated patient clinical data in small groups during supervised laboratory practicals in preparation for examination. At the end of such a course, there is an obligatory practical exam. For the spring 2006 Oral Pathology laboratory practicals, all the 50 slides previously unit of the laboratory practicals were digitized and placed on the web. Digitized slides were linked with macro images, radiological images, and patient case histories, all of which had been put on the web systematically since 1993 . Thus, the only variable in 2006 was adding the virtual slides to the web.
Criteria and methodology
Considering very good subjective as well as objective evaluation by our first “experimental” group in academic year 2005/2006, we have decided to go further and prove the usefulness of digitized slides to all students in the next years. Therefore, since academic year 2006/2007 we switched from traditional pathology microscope practicals to WebMicroscope-driven laboratory practicals in our third-year pathology course for all dental students. Additionally, we have experienced a growing number of students - from 64 in year 2005 to 116 in 2010. Instead of preparing additional glass slides for new students we have concentrated on completing and exchanging the existent glass slides with new cases. Not only all the slides used for laboratory practicals were completely digitized but we also added and/or replaced approximately 10 - 20 digitized slides every year. Virtual microscope WebMicroscope) was the only device used by the students during laboratory practicals conducted in up to 9 student groups. The laboratory practicals were supervised by staff and attendance was mandatory for the students. The evaluation system was based on credits obtained by the students during practicals and final examinations. In oral pathology, passing the practical exam was necessary to validate. Feedback from students was performed after the final examination.
Students evaluation and staff opinion
We undertook a comprehensive evaluation of the implementation of virtual microscopy at the end of every academic year. A questionnaire was distributed to all students who were asked to provide ratings from 1 (low) to 10 (high) for effectiveness, image quality, ease of use and usefulness of virtual slides. They were also asked to rate specifically the usefulness of practical laboratories in basic, systemic and oral pathology. In addition, space was provided for comments.
Usefulness of WebMicroscope was rated in:
n = 8 students
n = 84 students
n = 92 students
n = 102 students
n = 116 students
basic and systemic and oral pathology
mean = 9.4 ± 0.9
basic and systemic pathology
mean = 8.2 ± 1,9
basic and systemic pathology
mean = 7.9 ± 1.9
basic and systemic pathology
mean = 8.4 ± 2.4
basic and systemic pathology
mean = 7.8 ± 2.3
mean = 9.0 ± 1.5
mean = 8.2 ± 1.7
mean = 8.7 ± 2.3
mean = 8.8 ± 1.6
The staff valued the efficiency of the use of the WebMicroscope at the Department of Clinical Pathology, University of Medical Sciences in Poznan. This approach has allowed to analyze 8 – 12 slides per 2-hr class, with a wide-range discussion. Staff members were pleasantly surprised by the striking evidence of active and independent learning within practical classes, as well as the extent to which the students were willing to undertake collaborative group work. Moreover, many students asked questions seeking further details about pathological changes so that they could understand the relationship of morphological changes to the clinical manifestations of the disease. It was equally gratifying to see how well our students solved problems in understanding histopathological images when they learnt the relevant clinical data. In class, they often asked in-depth and thoughtful questions about how abnormalities that they identified developed.
Benefits of use of WebMicroscope
Although the major innovative aspect of virtual slide technology is that the computer workstation can emulate a traditional student microscope and glass slides, there are several other educational advantages of digitized slides. A major advantage is the efficiency and accessibility. The efficiency is due to the fact that students have all the slides accessible at a click at anytime and anywhere, in focus, with proper lighting and condenser adjustment, and a far superior quality in comparison with common quality of student light microscope. Slides are also annotated and integrated on-line with patient history, macro and radiological images, and linked with other web-based resources. This presents a markedly different experience from sitting at a traditional microscope with a photocopied paper data. Because the virtual slides are always in focus and with proper lighting, students can concentrate all their efforts on the content of the slide rather than the technical aspects of using a traditional microscope. Also, the single best slide from the existing collection or one available glass slide can be digitized and made available to all students at the same time. The interactions between students are enhanced. Several students can look at the same virtual slide image on a monitor and compare with their neighbors at an adjacent workstation, whereas a traditional laboratory may have predominantly single-headed microscopes. Already during the first laboratory practical held in the computer laboratory we observed a marked increase in the discussions among students as compared to the traditional practical.
As anticipated from the evaluation, students preferred the WebMicroscope-based laboratory practicals. The majority of students attended the scheduled practicals but many preferred additional access to the WebMicroscopy on their own outside the scheduled hours. Server log files indicated ca 8000 - 9000 home page hits per year.
The faculty benefit from virtual slide technology in that they can prepare, in their offices, for teaching in lab practicals and discussion groups, design virtual slides in lecture hall and laboratories, and can better interact with students as they observe virtual slides on the computer screen. The faculty can also easily download screen shots of virtual slides for use in other computer-based educational programs. The institution benefits because, as microscope laboratories are moved into computer rooms, will not have to support both computer laboratories and large expensive microscope laboratories.
We use the best currently available didactic tool - whole slide imaging (WSI), in order to prepare students to enter the mysterious world of diseased human cells and to succeed at their medical practice. Dental students have not only accepted this technology but have also shown enthusiasm for the development of further on-line resources for learning pathology. Our students now believe that working on their pathology skills using virtual microscopy will make them more knowledgeable and competitive in clinic, research, and dental practice. We believe this new cutting-edge virtual slide technology has also a significant potential for innovation beyond medical student and undergraduate education. This includes web-based publishing of atlases, textbooks and articles, continuing medical education, pathology resident education, learning / exploring the pathology of experimentally manipulated animals, proficiency testing, and certifying examinations.
Although the initial equipment and software cost for creating virtual slides is high, we believe that this new technology has a potential to revolutionize the way we teach and learn from microscopic images. Our long-term hope is that virtual slide technology will be promoted nationwide in the numerous innovative areas as those described above, so that it becomes an efficient and affordable technology for all. As teachers, using virtual microscopy we can get more information about how our students learn pathology, how instructive the laboratory practicals are and which slides are of real didactic value for the students.
The authors thank the WebMicroscope Team at the University of Helsinki for their valuable and friendly support, dental students of the years 2005 - 2010 for their cooperation in this experiment, as well as associate professors, adjuncts and assistants of the Chair of Clinical Pathology University of Medical Sciences in Poznan who have been teaching the students using the WebMicroscope in 2006-2010 and who gave constructive criticism for this paper.
This article has been published as part of Diagnostic Pathology Volume 6 Supplement 1, 2011: Proceedings of the 10th European Congress on Telepathology and 4th International Congress on Virtual Microscopy. The full contents of the supplement are available online at http://www.diagnosticpathology.org/supplements/6/S1
- Dee FR: Virtual microscopy in pathology education. Hum Pathol. 2009, 40: 1112-1121. 10.1016/j.humpath.2009.04.010.View ArticlePubMed
- Lundin M, Lundin J, Isola J: Virtual microscopy. J Clin Pathol. 2004, 57: 1250-1251. 10.1136/jcp.2004.019919.PubMed CentralView ArticlePubMed
- Glatz-Krieger K, Spornitz U, Spatz A, Mihatsch MJ, Glatz D: Factors to keep in mind when introducing virtual microscopy. Virchows Arch. 2006, 448: 248-255. 10.1007/s00428-005-0112-2.View ArticlePubMed
- Szymas J, Gawronski M: Multimedial data base and management system for self-education and testing the students' knowledge on pathomorphology. Patol Pol. 1993, 44: 183-187.PubMed
- Cotter JR: Laboratory instruction in histology at the University at Buffalo: recent replacement of microscope exercises with computer applications. Anat Rec. 2001, 265: 212-221. 10.1002/ar.10010.View ArticlePubMed
- Brisbourne MA, Chin SS, Melnyk E, Begg DA: Using Web-based animations to teach histology. Anat Rec. 2002, 269: 11-9. 10.1002/ar.10054.View ArticlePubMed
- Gona AG, Berendsen PB, Alger EA: New approach to teaching histology. JIAMSE. 2005, 15: 57-59.
- Harris T, Leaven T, Heidger P, Kreiter C, Duncan J, Dick F: Comparison of a virtual microscope laboratory to a regular microscope laboratory for teaching histology. Anat Rec. 2001, 265: 10-14. 10.1002/ar.1036.View ArticlePubMed
- Heidger P, Dee FR, Consoer D, Leaven T, Kreiter C: An integrated approach to teaching and testing in histology with real and virtual imaging. Anat Rec. 2002, 269: 107-12. 10.1002/ar.10078.View ArticlePubMed
- Blake CA, Lavoie HA, Millette CF: Teaching medical histology at the University of South Carolina School of Medicine: transition to virtual slides and virtual microscopes. Anat Rec. 2003, 275: 196-206. 10.1002/ar.b.10037.View Article
- Krippendorf BB, Lough J: Complete and rapid switch from light microscopy to virtual microscopy for teaching medical histology. Anat Rec. 2005, 285: 19-25. 10.1002/ar.b.20066.View Article
- Bloodgood RA, Ogilvie RW: Trends in histology laboratory teaching in United States medical schools. Anat Rec. 2006, 289B: 169-75. 10.1002/ar.b.20111.View Article
- Ogilvie RW, Groscurth P, Rohr H, Benecke H, Rohr B, Konig R: WebMic, a virtual microscope for learning histology: results of implementation in medical histology course. Virtual microscopy and virtual slides in teaching, diagnosis and research. Edited by: Gu J, Ogilvie RW. 2005, New York: Taylor and Francis, 161-175. full_text.View Article
- Kumar RK, Velan GM, Korell SO, Kandara M, Dee FR, Wakefield D: Virtual microscopy for learning and assessment in pathology. J Pathol. 2004, 204: 613-618. 10.1002/path.1658.View ArticlePubMed
- Farah C, Maybury T: Implementing digital technology to enhance students learning pathology. Eur J Dent Educ. 2009, 13: 172-178. 10.1111/j.1600-0579.2009.00570.x.View ArticlePubMed
- Grabe N, Schirmacher P: From virtual microscopy to systems pathology. Virchow Arch. 2009, 455: 193-196. 10.1007/s00428-009-0810-2.View Article
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.