Estrogen-producing endometrioid adenocarcinoma resembling sex cord-stromal tumor of the ovary: a review of four postmenopausal cases
© Katoh et al.; licensee BioMed Central Ltd. 2012
Received: 15 September 2012
Accepted: 14 November 2012
Published: 28 November 2012
The 4 present cases with endometrioid adenocarcinoma (EMA) of the ovary were characterized by estrogen overproduction and resemblance to sex cord-stromal tumor (SCST). The patients were all postmenopausal, at ages ranging from 60 to 79 years (av. 67.5), who complained of abdominal discomfort or distention and also atypical genital bleeding. Cytologically, maturation of the cervicovaginal squamous epithelium and active endometrial proliferation were detected. The serum estrogen (estradiol, E2) value was preoperatively found to be elevated, ranging from 48.7 to 83.0 pg/mL (av. 58.4). In contrast, follicle stimulating hormone was suppressed to below the normal value. MR imaging diagnoses included SCSTs such as granulosa cell tumor or thecoma for 3 cases because of predominantly solid growth, and epithelial malignancy for one case because of cystic and solid structure. Grossly, the solid part of 3 cases was homogeneously yellow in color. Histologically, varying amounts of tumor components were arranged in solid nests, hollow tubules, cord-like strands and cribriform-like nests in addition to the conventional EMA histology.
In summary, postmenopausal ovarian solid tumors with the estrogenic manifestations tend to be preoperatively diagnosed as SCST. Due to this, in the histological diagnosis, this variant of ovarian EMA may be challenging and misdiagnosed as SCST because of its wide range in morphology.
KeywordsOvary Postmenopausal Estrogen (E2) overproduction Endometrioid adenocarcinoma Resembling sex cord-stromal tumor
Endometrioid adenocarcinoma (EMA) may occur as a unique variant, irrespective being of ovarian origin or uterine endometrial origin . The patients with ovarian tumors often complain variable and unusual symptoms . Some postmenopausal patients with ovarian tumor present with atypical genital bleeding. In addition to this symptom, when endometrial thickening is detected on the imaging examination, the possibility of estrogen overproduction by the ovarian tumor can be raised. Cervicovaginal cytology also shows an increased maturation of squamous epithelium due to estrogenic effects . As one of the most representative ovarian tumors with estrogen overproduction in postmenopausal women, adult granulose cell tumor is routinely encountered. Theco-fibromatous tumor and Brenner tumor arising in postmenopausal women are also known as having a potential to produce estrogen more than the normal range.
Since 3 to 4 decades ago, postmenopausal epithelial ovarian tumors have been found to overproduce estrogen with considerable frequency [4, 5]. According to the certain reports, mucinous tumor is most frequently characterized by estrogen overproduction [6–8]. In the other histological types such as serous, endometrioid and clear cell tumors, however, the recognition for their potential to overproduce estrogen seems to be less generalized, not only for pathologists but also for gynecologists.
In our institution, endocrinological examination including the serum value of estrogen (E2) and follicle stimulating hormone (FSH) was performed for solid ovarian tumors arising in postmenopausal women, especially when they present with atypical genital bleeding, endometrial thickening and/or an increased maturation of squamous epithelium. As a result, 6 cases with ovarian EMA having E2 overproduction were encountered during the last 5 years in our institution. Among them, 4 cases were diagnosed as EMA resembling sex cord-stromal tumor (SCST). In addition to EMA, a case with serous adenocarcinoma and 3 cases with clear cell adenocarcinoma which showed estrogen overproduction were also experienced. Based on the histological observation alone, it is less easy to determine whether or not the ovarian epithelial tumors may overproduce estrogen. As a tumor marker in the ovarian tumors, estrogen may have a clinically significant implication [5–7].
atypical genital bleeding
atypical genital bleeding
a E2 (pg/ml)
a FSH (mIU/ml)
GCT or thecoma
GCT or thecoma
thecoma, fibroma or Brenner tumor
TAH, BSO, OMT
TAH, BSO, OMT, TCx6
NED 48 months
NED 38 months
NED 7 months
NED 39 months
solid and cystic/gray-white
cervicovaginal smear (MI)
EMAF and MCA
The endocervical glands of the hysterectomy specimens were more activated in the secretion compared to those in postmenopausal patients with non-estrogen producing ovarian tumor. The endometrial glands seemed to be functioning irrespective of the patients’ postmenopausal status. These endocervical and endometrial findings were in concordance with the cytological findings of the cervicovaginal smear and the endometrial smear, respectively.
The FIGO stage was determined at IA for Cases 1, 2, and 3, and IC for Case 4.
Ovarian EMA has been known for approximately 30 years to have a histological variant which is characterized by a resemblance to SCST [12, 13], but the precise frequency of EMA resembling SCST remains to be clarified. According to Young et al., 8 of 13 cases were initially misdiagnosed as SCST . A lack of accurate recognition about this variant may yield a misinterpretation which results in the misdiagnosis as SCST instead of EMA. Especially, at the intraoperative diagnosis, both the macroscopic and microscopic findings may mislead pathologists to raise the possibility of SCST. Although our experience is limited, the 4 present cases were encountered during the last 5 years since the opening of our institution, accounting for approximately 10% of the total ovarian EMAs. In fact, Case 1 (Figure 3a, b) was initially diagnosed by the referring pathologist as Sertoli-Leydig cell tumor with estrogen overproduction, mainly because of his misinterpreting the luteinized stromal cells as Leydig cells. One of the critical differential points between Sertoli-Leydig cell tumor and EMA is the occurrence age: 28 years for the former and over 55 years for the latter . But, interestingly, it should be noted that some Sertoli-Leydig cell tumors contain areas which resemble endometrioid tumor . As for Case 2 (Figure 3c, d), at the intraoperative diagnosis, the possible diagnosis of Sertoli cell tumor was made by the referring pathologist. In this case, the presence of granulosa cell tumor-like components also may have become a key to misdiagnosis.
It has not been pathologically defined as to what part of the components resembling SCST is supposed to be contained for the diagnosis of this variant. Reportedly, the proportions of components resembling SCST in the entire tumor vary considerably, ranging from 30 to 100% . In our review, too, the proportions ranged from 20 to 80%. Except for EMA which is almost entirely occupied by the components resembling SCST, as in Case 3 (Figure 3e, f, g), a combination with a variable amount of conventional EMA would favor the diagnosis of EMA resembling SCST. Though relatively rare, a coexistence of cribriform-like nests and solid nests may make it difficult to lead to the accurate diagnosis of a variant of EMA. In addition, the histologically reliable hallmarks of EMA are as follows: squamous differentiation of the tumor cells, complication of endometriosis within the tumor or in the background, and transition between adenofibromatous components of endometrioid type and carcinoma . In Case 2, endometriosis was speculated to be associated with tumor development. In Case 4 (Figure 3h, i, j), a transition with the benign component of adenofibromatous endometrioid tumor was considered to be strongly supportive of the diagnosis of a variant of EMA. But, it should be noted that mucinous cystadenoma may coexist not only in other epithelial tumors but also in SCSTs. Interestingly, in this case, the stromal cells were found to be more cellular in the vicinity of mucinous cystadenoma rather than within the endometrioid benign and malignant tumors.
When histologically challenging cases are encountered, certain immunohistochemical markers are thought to contribute greatly to making the diagnosis definite. The epithelial nature of EMA, in spite of the resemblance to SCST, could be determined by positive reactions for CK7 and epithelial membrane antigen [15–17]. Many EMAs are positive for ER  but inconsistently negative for inhibin-α [15, 17]. In the cases with pure SCST, inhibin-α is frequently expressed, while these epithelial markers are less frequently or weakly expressed if at all [15–17], and ER expression is usually negative .
The first descriptions about the ovarian functioning stroma go far back to the 1950s . This stromal change has been considered to be closely associated with clinical, biochemical or pathological evidence of endocrine function [4–7]. Several investigators demonstrated that many postmenopausal women with a variable epithelial ovarian tumor would be provided with a potential to produce more sex steroid hormone represented by estrogen than normal postmenopausal women [6–8, 20, 21]. The frequency of estrogen overproduction is reported to be over 70% by serological examinations [7, 8], and 47%  or 50%  by urine analyses. According to some reports, steroidogenic cells are observed more frequently in mucinous tumors [6, 8, 20], and predominantly in postmenopausal women (80%) . However, the difference in the incidence of estrogen overproduction among the variable types of ovarian tumors remains controversial . Concerning the difference among benign tumor, borderline malignancy, and malignant tumor, the previous reports do not seem to present confirmative data because of their statistically small scale of examined cases as well as their deviation in histological types [6–8, 20, 21].
In the report by Young et al., the association between EMA resembling SCST and estrogenic manifestation or presence of steroidogenic stroma was described as follows: 4 of 13 cases had the stroma consistent with that of luteinized stromal cells, but the evidence of possible estrogen overproduction was noted in only one case . There is another description that theca-like stroma and foci of vacuolated lutein-like cells were noted in one of 4 cases with EMA . To the best of our knowledge, with regard to postmenopausal EMA with estrogen overproduction which was confirmed by serological examination, there have been only 4 reported cases [11, 23–25]. These 4 cases included one case with EMA resembling SCST, and the remaining 3 cases showed conventional EMA. Based on our limited experience, it is speculated that EMA resembling SCST may have more estrogenic manifestations due to the presence of steroidogenic stroma. Focusing on the source of estrogen overproduction and its mechanism, in terms of the morpho-functional correlation, immunohistochemical and molecular-based analyses have been performed [9–11, 23, 26, 27]. Interestingly, Sasano et al. mentioned that SF-1 and steroidogenic enzymes are expressed in not only stromal cells but also epithelial tumor cells . However, the association between clinical hormonal abnormalities and presence or absence of luteinized stromal cells in ovarian tumor, including the relationship with the mechanism of extragonadal aromatization, has not been fully established yet .
In summary, EMA resembling SCST should be definitely differentiated from SCST from the clinical and pathological aspects. In order to clarify the significance of estrogen as a tumor marker for the ovarian tumor and its utility in the management of the patient, more precise clinical and pathological data must be gathered. Furthermore, the molecular-based mechanism with estrogen overproduction needs to be explored.
- Singh K, Simon RA, Zhang C, Quddus MR: "Surface epithelial changes" in uterine endometrioid carcinoma mimicking micropapillary serous borderline tumor of ovary: report of two cases and review of the literature. Diagn Pathol. 2011, 6: 13-10.1186/1746-1596-6-13.PubMed CentralView ArticlePubMedGoogle Scholar
- Tomas D, Lenicek T, Tuckar N, Puljiz Z, Ledinsky M, Kruslin B: Primary ovarian leiomyoma associated with endometriotic cyst presenting with symptoms of acute appendicitis: a case report. Diagn Pathol. 2009, 4: 25-10.1186/1746-1596-4-25.PubMed CentralView ArticlePubMedGoogle Scholar
- Rubin DK, Frost JK: The cytologic detection of ovarian cancer. Acta Cytol. 1963, 7: 191-195.PubMedGoogle Scholar
- Rome RM, Laverty CR, Brown JB: Ovarian tumours in postmenopausal women. Clinicopathological features and hormonal studies. J Obstet Gynaecol Br Commonw. 1973, 80: 984-991. 10.1111/j.1471-0528.1973.tb02961.x.View ArticlePubMedGoogle Scholar
- Rome RM, Fortune DW, Quinn MA, Brown JB: Functioning ovarian tumors in postmenopausal women. Obstet Gynecol. 1981, 57: 705-710.PubMedGoogle Scholar
- Suzuki M, Sekiguchi I, Tamada T: Estrogen production by epithelial ovarian tumors and dermoid cyst. Acta Obst Gynaec Jpn. 1986, 38: 303-310. in JapaneaseGoogle Scholar
- Obata N, Sato Y, Tanaka K, Sasaki A, Takeuchi S, Hanaoka J, et al.: Pathological and endocrinological study of epithelial ovarian tumors in post-menopausal women. Acta Obst Gynaec Jpn. 1986, 38: 647-656. in JapaneaseGoogle Scholar
- Yamagata S, Yamamoto K, Tsuchida S, Kawamura N, Matsumoto Y, Ueki S, et al.: Estrogen production in epithelial tumors of the ovary: clinical and endocrinological study in postmenopausal women. Acta Obst Gynaec Jpn. 1989, 41: 1761-1768. in JapaneaseGoogle Scholar
- Sasano H, Kaga K, Sato S, Yajima A, Nagura H: Adrenal 4-binding protein in common epithelial and metastatic tumors of the ovary. Hum Pathol. 1996, 27: 595-598. 10.1016/S0046-8177(96)90168-9.View ArticlePubMedGoogle Scholar
- Abd-Elaziz M, Moriya T, Akahira J, Nakamura Y, Suzuki T, Sasano H: Immunolocalization of nuclear transcription factors, DAX-1 and Ad4BP/SF-1, in human common epithelial ovarian tumors: correlations with StAR and steroidogenic enzymes in epithelial ovarian carcinoma. Int J Gynecol Pathol. 2005, 24: 153-163. 10.1097/01.pgp.0000155075.75209.42.View ArticlePubMedGoogle Scholar
- Tokunaga H, Akahira J, Suzuki T, Moriya T, Sasano H, Ito K, et al.: Ovarian epithelial carcinoma with estrogen-producing stroma. Pathol Int. 2007, 57: 285-290. 10.1111/j.1440-1827.2007.02099.x.View ArticlePubMedGoogle Scholar
- Young RH, Prat J, Scully RE: Ovarian endometrioid carcinomas resembling sex cord-stromal tumors. A clinicopathological analysis of 13 cases. Am J Surg Pathol. 1982, 6: 513-522. 10.1097/00000478-198209000-00003.View ArticlePubMedGoogle Scholar
- Roth LM, Liban E, Czernobilsky B: Ovarian endometrioid tumors mimicking Sertoli and Sertoli-Leydig cell tumors: Sertoliform variant of endometrioid carcinoma. Cancer. 1982, 50: 1322-1331. 10.1002/1097-0142(19821001)50:7<1322::AID-CNCR2820500718>3.0.CO;2-C.View ArticlePubMedGoogle Scholar
- McCluggage WG, Young RH: Ovarian sertoli-leydig cell tumors with pseudoendometrioid tubules (pseudoendometrioid sertoli-leydig cell tumors). Am J Surg Pathol. 2007, 31: 592-597. 10.1097/01.pas.0000213365.56498.72.View ArticlePubMedGoogle Scholar
- Ordi J, Schammel DP, Rasekh L, Tavassoli FA: Sertoliform endometrioid carcinomas of the ovary: a clinicopathologic and immunohistochemical study of 13 cases. Mod Pathol. 1999, 12: 933-940.PubMedGoogle Scholar
- Aguirre P, Thor AD, Scully RE: Ovarian endometrioid carcinomas resembling sex cord-stromal tumors. An immunohistochemical study. Int J Gynecol Pathol. 1989, 8: 364-373. 10.1097/00004347-198912000-00008.View ArticlePubMedGoogle Scholar
- Guerrieri C, Franlund B, Malmstrom H, Boeryd B: Ovarian endometrioid carcinomas simulating sex cord-stromal tumors: a study using inhibin and cytokeratin 7. Int J Gynecol Pathol. 1998, 17: 266-271. 10.1097/00004347-199807000-00012.View ArticlePubMedGoogle Scholar
- Miyazawa M, Yasuda M, Fujita M, Hirabayashi K, Hirasawa T, Kajiwara H, et al.: Granulosa cell tumor with activated mTOR-HIF-1alpha-VEGF pathway. J Obstet Gynaecol Res. 2010, 36: 448-453. 10.1111/j.1447-0756.2009.01127.x.View ArticlePubMedGoogle Scholar
- Morris JM, Scully RE: Endocrine Pathology of the Ovary. 1958, St Louis: CV Mosby, 131-139.Google Scholar
- Ishikura H, Sasano H: Histopathologic and immunohistochemical study of steroidogenic cells in the stroma of ovarian tumors. Int J Gynecol Pathol. 1998, 17: 261-265. 10.1097/00004347-199807000-00011.View ArticlePubMedGoogle Scholar
- Rutgers JL, Scully RE: Functioning ovarian tumors with peripheral steroid cell proliferation: a report of twenty-four cases. Int J Gynecol Pathol. 1986, 5: 19-337.View ArticleGoogle Scholar
- Wren BG, Frampton J: Oestrogenic activity associated with non-feminizing ovarian tumours after the menopause. Br Med J. 1963, 2: 842-844. 10.1136/bmj.2.5361.842.PubMed CentralView ArticlePubMedGoogle Scholar
- Kurihara Y, Kasahara K, Sasano H, Tsutsumi Y: Ovarian endometrioid adenocarcinoma associated with steroidogenic foamy stromal cells. Pathol Int. 1997, 47: 883-888. 10.1111/j.1440-1827.1997.tb03722.x.View ArticlePubMedGoogle Scholar
- Hayasaka T, Nakahara K, Kojimahara T, Saito-Sekiguchi M, Motoyama T, Kurachi H: Endometrioid adenocarcinoma with a functioning stroma. J Obstet Gynaecol Res. 2007, 33: 381-383. 10.1111/j.1447-0756.2007.00541.x.View ArticlePubMedGoogle Scholar
- Ouchi Y, Matsumoto S, Aoki M, Kamihara Y, Kobata K, Ohgami A, et al.: Ovarian endometrioid carcinoma resembling sex cord-stromal tumors: a case report. Kyushu Bran Jap Soc Clin Cytol. 2010, 41: 89-94. in JapaneaseGoogle Scholar
- Matias-Guiu X, Prat J: Ovarian tumors with functioning stroma. An immunohistochemical study of 100 cases with human chorionic gonadotropin monoclonal and polyclonal antibodies. Cancer. 1990, 65: 2001-2005. 10.1002/1097-0142(19900501)65:9<2001::AID-CNCR2820650920>3.0.CO;2-7.View ArticlePubMedGoogle Scholar
- Yasuda M, Itoh J, Hirasawa T, Hirazono K, Shinozuka T, Sasano H, et al.: Serous borderline ovarian tumor with functioning stroma in a postmenopausal woman: immunohistochemical analysis of steroidogenic pathway. Int J Gynecol Pathol. 1998, 17: 75-78. 10.1097/00004347-199801000-00013.View ArticlePubMedGoogle Scholar
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