Paratesticular dedifferentiated liposarcoma with leiomyosarcomatous differentiation: a case report with a review of literature
© Hatanaka et al.; licensee BioMed Central Ltd. 2013
Received: 25 July 2013
Accepted: 15 August 2013
Published: 23 August 2013
Paratesticular liposarcoma is a rare neoplasm, described in single case studies or components of larger studies, as histologically well-differentiated liposarcoma (WDL) and dedifferentiated liposarcoma (DL). However, leiomyosarcomatous differentiation is an extremely rare occurrence in WDL and DL. We report a case of leiomyosarcomatous differentiation in a 77-year-old man. The patient presented with a painless right scrotal mass. Magnetic resonance imaging showed a large mass along the right spermatic cord. The resected mass, measuring 17.5 × 12 × 5 cm, was composed of a high-grade pleomorphic undifferentiated sarcomatous component with necrosis. Atypical smooth muscle differentiation was also detected. Additional tumor sampling revealed the presence of a WDL component. Immunohistochemical analysis of the pleomorphic sarcomatous component showed positive staining for MDM2 and CDK4, and negative staining for alpha smooth muscle actin (αSMA) and desmin. The smooth muscle component was positive for αSMA and desmin, and negative for MDM2 and CDK4. Extension from primary retroperitoneal sarcoma was not proved. We diagnosed of DL with leiomyosarcomatous differentiation.
The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1484291498104021.
KeywordsDedifferentiated liposarcoma Leiomyosarcomatous differentiation Paratesticular region
Liposarcoma is the most common type of soft-tissue sarcoma, accounting for about 20% of all mesenchymal malignant tumors. It usually arises from the thigh and retroperitoneum. However, pleomorphic liposarcomas arising in the foot and ankle have been reported . The occurrence of liposarcomas in the paratesticular regions is also rare . A study of 30 cases of paratesticular liposarcoma showed that 19 cases were well-differentiated liposarcoma (WDL), 10 cases were dedifferentiated liposarcoma (DL), and 1 showed a myxoid/round cell liposarcoma . DL is usually composed of atypical lipomatous tumor (ALT)/WDL areas and dedifferentiated components that usually overlap with spindle/pleomorphic cell high-grade sarcoma or myxoid/spindle cell low-grade sarcoma. Dedifferentiated areas rarely show heterologous differentiation with myogenic, osteo/chondrosarcomatous, or angiosarcomatous elements . Moreover, the peculiar meningothelial-like whorling and metaplastic bone formation were reported as other elements of heterologous differentiation . Leiomyosarcoma, rhabdomyosarcoma, chondrosarcoma, and osteosarcoma differentiation has also been reported in malignant mesenchymal tumor .
Paratesticular liposarcoma with leiomyosarcomatous differentiation is extremely rare; to our knowledge, only single case studies or components of larger studies have been reported in the English literature [6–9]. Here, we report a very rare case of paratesticular DL with leiomyosarcomatous differentiation with a review of literature.
A 77-year-old man with diabetes mellitus presented with a growing mass in the right testis of 3 months duration. A computed tomographic scan revealed a soft tissue mass, measuring 12 cm in diameter, in the right scrotal sac. The mass showed low signal intensities on T2-weighted magnetic resonance imaging. Radical inguinal orchiectomy was performed because of suspected malignancy.
Materials and methods
The resected specimens were fixed with 10% neutral-buffered formalin and embedded in paraffin blocks. Four micrometer-thick paraffin sections were cut and mounted on glass slides, deparaffinized in xylene, rehydrated with ethanol, and immunostained with the following antibodies: MDM2 (monoclonal, 1B10, Novocastra, Newcastle upon Tyne, UK), CDK4 (monoclonal, DCS-31, Invitrogen, Camarillo, CA), S-100 protein (polyclonal, Z0311, Dako, Glostrup, Denmark), alpha smooth muscle actin (αSMA) (monoclonal, αsm-1, Novocastra), desmin (monoclonal, D33, Dako, Glostrup, Denmark), and MyoD1 (monoclonal, 5.8A, Dako, Glostrup, Denmark). Sections were stained using Dako Envision kit (Dako, Glostrup, Denmark).
DL with leiomyosarcomatous differentiation was diagnosed. The leiomyosarcomatous component occupied approximately 10% of the tumor. The patient was disease free after at the 2-year follow-up.
Paratesticular liposarcoma is rare and has been reported in single case reports or as components of larger series. These tumors are thought to arise de novo from the adipose tissue around the spermatic cord or by malignant transformation of pre-existing lipomas . In 2003, Montogomery et al studied 30 cases of paratesticular liposarcomas in men aged 41 – 87 years (mean age, 63 years) . The tumors involved the spermatic cord (23 cases), testicular tunics (6 cases), or epididymis (1 case) and measured from 3 - 30 cm (mean, 11.7 cm). From the histological viewpoint, 19 cases were WDL, 10 were DL, and 1 was a myxoid/round cell liposarcoma. Among 10 DL cases, 5 were high-grade and 5 were low-grade. Leiomyosarcomatous differentiation was not described in this series. Binh et al reported 27 cases of DL with divergent myosarcomatous differentiation, including 7 cases in the paratesticular area, and leiomyosarcomatous differentiation was detected in 7 cases . Henricks et al reported 155 cases of DL, including 13 cases of spermatic cord/scrotum origin, and leiomyosarcomatous differentiation was observed in 2 of the 155 cases . However, the relationship between the tumor location and the histological findings was not available in both studies.
Clinicopathological features of well-documented cases of paratesticular liposarcoma with leiomyosarcomatous differentiation
Classification of liposarcoma
Smooth muscle component
It is important to recognize the coexistence of the ALT/WDL components in order to obtain a definitive diagnosis of DL. However, if these components are not recognized, the differential diagnosis includes a variety of lesions, benign or low-grade mesenchymal tumors for low-grade differentiation, and pleomorphic sarcomas for high-grade differentiation. The general differential diagnosis of adult spindle cell tumors in this site includes primarily fibrous mesothelioma, leiomyosarcoma, malignant fibrous histiocytoma, various benign fibrous tumors, pseudotumors, and fibromatosis . In addition to morphological findings, immunohistochemical study, including MDM2 and CDK4 staining, may be useful for diagnosis. The main karyotypic alteration of DL is considered to be the presence of supernumerary ring chromosomes and/or giant chromosomes composed of a chromosome 12q13-15 sequence involving MDM2 and often CDK4 genes . The utility of MDM2 and CDK4 immunostaining is well documented in the diagnosis of WDL and DL . However, MDM2 and CDK4 are not specific markers for liposarcoma. In the present case, the leiomyosarcomatous component showed no immunopositivity for MDM2 and CDK4; however, some leiomyosarcomas show positivity for MDM2 and CDK4 . Therefore, it is important to prove the existence of the ALT/WDL component through additional sampling of the tumor.
Clinically, the risk of local recurrence is not affected by myosarcomatous differentiation in DL, although the metastatic rate is relatively low compared to that of leiomyosarcoma. The prognosis of patients with DL with myosarcomatous differentiation is better than that of patients with leiomyosarcoma . Therefore, from a clinical perspective, it is important to distinguish DL with myosarcomatous differentiation from leiomyosarcoma in an argument for conventional adjuvant chemotherapy.
In summary, we report a rare case of paratesticular DL with with leiomyosarcomatous differentiation in a 77-year-old man. This case highlights the importance of additional sampling for obtaining a definitive diagnosis of DL, especially if the existence of an ALT/WDL component is not noted.
Written informed consent was obtained from the patient for publication of this case report and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.
We thank Dr. Masanori Hisaoka, Department of Pathology and Oncology, School of Medicine, University of Occupational and Environmental Health, for his thoughtful comments on the pathological diagnosis. This work was supported by a grant from the Kodama Memorial Fund for Medical Research.
- Brcić L, Jakovcević A, Vuletić LB, Orlić D, Seiwerth S: Pleomorphic liposarcoma of the foot: a case report. Diagn Pathol. 2008, 3: 15-10.1186/1746-1596-3-15.PubMed CentralView ArticlePubMed
- Montgomery E, Fisher C: Paratesticular liposarcoma: a clinicopathologic study. Am J Surg Pathol. 2003, 27: 40-47. 10.1097/00000478-200301000-00005.View ArticlePubMed
- Dei Tos AP: Liposarcoma: new entities and evolving concepts. Ann Diagn Pathol. 2000, 4: 252-266. 10.1053/adpa.2000.8133.View ArticlePubMed
- Song JS, Gardner JM, Tarrant WP, Shen S, Ayala AG, Yu E, Ro JY: Dedifferentiated liposarcoma with peculiar meningothelial-like whorling and metaplastic bone formation. Ann Diagn Pathol. 2009, 13: 278-284. 10.1016/j.anndiagpath.2008.07.002.View ArticlePubMed
- Li YF, Yu CP, Wu ST, Dai MS, Lee HS: Malignant mesenchymal tumor with leiomyosarcoma, rhabdomyosarcoma, chondrosarcoma, and osteosarcoma differentiation: case report and literature review. Diagn Pathol. 2011, 6: 35-10.1186/1746-1596-6-35.PubMed CentralView ArticlePubMed
- Evans HL: Smooth muscle in atypical lipomatous tumors. Am J Surg Pathol. 1990, 14: 714-718. 10.1097/00000478-199008000-00002.View ArticlePubMed
- Suster S, Wong TY, Moran CA: Sarcomas with combined features of liposarcoma and leiomyosarcoma: study of two cases of an unusual soft-tissue tumor showing dual lineage differentiation. Am J Surg Pathol. 1993, 17: 905-911. 10.1097/00000478-199309000-00006.View ArticlePubMed
- Flope AL, Weiss SW: Lipoleiomyosarcoma (well-differentiated liposarcoma with leiomyosarcomatous differentiation): a clinicopathologic study of nine cases including one with dedifferentiation. Am J Surg Pathol. 2002, 26: 742-749. 10.1097/00000478-200206000-00007.View Article
- Binh MB, Guillou L, Hostein I, Château MC, Collin F, Aurias A, Binh BN, Stoeckle E, Coindre JM: Dedifferentiated liposarcomas with divergent myosarcomatous differentiation developed in the internal trunk: a study of 27 cases and comparison to conventional dedifferentiated liposarcomas and leiomyosarcomas. Am J Surg Pathol. 2007, 31: 1557-1566. 10.1097/PAS.0b013e31804b4109.View ArticlePubMed
- Khoubehi B, Mishra V, Ali M, Motiwala H, Karim O: Adult paratesticular tumours. BJU Int. 2002, 90: 707-715. 10.1046/j.1464-410X.2002.02992.x.View ArticlePubMed
- Henricks WH, Chu YC, Goldblum JR, Weiss SW: Dedifferentiated liposarcoma: a clinicopathological analysis of 155 cases with a proposal for an expanded definition of dedifferentiation. Am J Surg Pathol. 1997, 21: 271-281. 10.1097/00000478-199703000-00002.View ArticlePubMed
- Pedeutour F, Forus A, Coindre JM, Berner JM, Nicolo G, Michiels JF, Terrier P, Ranchere-Vince D, Collin F, Myklebost O, Turc-Carel C: Structure of the supernumerary ring and giant rod chromosomes in adipose tissue tumors. Genes chromosomes. Cancer. 1999, 24: 30-41.
- Binh MB, Sastre-Garau X, Guillou L, de Pinieux G, Terrier P, Lagacé R, Aurias A, Hostein I, Coindre JM: MDM2 and CDK4 immunostainings are useful adjuncts in diagnosing well-differentiated and dedifferentiated liposarcoma subtypes: a comparative analysis of 559 soft tissue neoplasms with genetic data. Am J Surg Pathol. 2005, 29: 1340-1347. 10.1097/01.pas.0000170343.09562.39.View ArticlePubMed
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.