Paratesticular dedifferentiated liposarcoma with leiomyosarcomatous differentiation: a case report with a review of literature

  • Kazuhito Hatanaka1,

    Affiliated with

    • Takako Yoshioka1,

      Affiliated with

      • Takashi Tasaki1 and

        Affiliated with

        • Akihide Tanimoto1Email author

          Affiliated with

          Diagnostic Pathology20138:142

          DOI: 10.1186/1746-1596-8-142

          Received: 25 July 2013

          Accepted: 15 August 2013

          Published: 23 August 2013

          Abstract

          Paratesticular liposarcoma is a rare neoplasm, described in single case studies or components of larger studies, as histologically well-differentiated liposarcoma (WDL) and dedifferentiated liposarcoma (DL). However, leiomyosarcomatous differentiation is an extremely rare occurrence in WDL and DL. We report a case of leiomyosarcomatous differentiation in a 77-year-old man. The patient presented with a painless right scrotal mass. Magnetic resonance imaging showed a large mass along the right spermatic cord. The resected mass, measuring 17.5 × 12 × 5 cm, was composed of a high-grade pleomorphic undifferentiated sarcomatous component with necrosis. Atypical smooth muscle differentiation was also detected. Additional tumor sampling revealed the presence of a WDL component. Immunohistochemical analysis of the pleomorphic sarcomatous component showed positive staining for MDM2 and CDK4, and negative staining for alpha smooth muscle actin (αSMA) and desmin. The smooth muscle component was positive for αSMA and desmin, and negative for MDM2 and CDK4. Extension from primary retroperitoneal sarcoma was not proved. We diagnosed of DL with leiomyosarcomatous differentiation.

          Virtual slides

          The virtual slide(s) for this article can be found here: http://​www.​diagnosticpathol​ogy.​diagnomx.​eu/​vs/​1484291498104021​.

          Keywords

          Dedifferentiated liposarcoma Leiomyosarcomatous differentiation Paratesticular region

          Background

          Liposarcoma is the most common type of soft-tissue sarcoma, accounting for about 20% of all mesenchymal malignant tumors. It usually arises from the thigh and retroperitoneum. However, pleomorphic liposarcomas arising in the foot and ankle have been reported [1]. The occurrence of liposarcomas in the paratesticular regions is also rare [2]. A study of 30 cases of paratesticular liposarcoma showed that 19 cases were well-differentiated liposarcoma (WDL), 10 cases were dedifferentiated liposarcoma (DL), and 1 showed a myxoid/round cell liposarcoma [2]. DL is usually composed of atypical lipomatous tumor (ALT)/WDL areas and dedifferentiated components that usually overlap with spindle/pleomorphic cell high-grade sarcoma or myxoid/spindle cell low-grade sarcoma. Dedifferentiated areas rarely show heterologous differentiation with myogenic, osteo/chondrosarcomatous, or angiosarcomatous elements [3]. Moreover, the peculiar meningothelial-like whorling and metaplastic bone formation were reported as other elements of heterologous differentiation [4]. Leiomyosarcoma, rhabdomyosarcoma, chondrosarcoma, and osteosarcoma differentiation has also been reported in malignant mesenchymal tumor [5].

          Paratesticular liposarcoma with leiomyosarcomatous differentiation is extremely rare; to our knowledge, only single case studies or components of larger studies have been reported in the English literature [69]. Here, we report a very rare case of paratesticular DL with leiomyosarcomatous differentiation with a review of literature.

          Case presentation

          A 77-year-old man with diabetes mellitus presented with a growing mass in the right testis of 3 months duration. A computed tomographic scan revealed a soft tissue mass, measuring 12 cm in diameter, in the right scrotal sac. The mass showed low signal intensities on T2-weighted magnetic resonance imaging. Radical inguinal orchiectomy was performed because of suspected malignancy.

          Materials and methods

          The resected specimens were fixed with 10% neutral-buffered formalin and embedded in paraffin blocks. Four micrometer-thick paraffin sections were cut and mounted on glass slides, deparaffinized in xylene, rehydrated with ethanol, and immunostained with the following antibodies: MDM2 (monoclonal, 1B10, Novocastra, Newcastle upon Tyne, UK), CDK4 (monoclonal, DCS-31, Invitrogen, Camarillo, CA), S-100 protein (polyclonal, Z0311, Dako, Glostrup, Denmark), alpha smooth muscle actin (αSMA) (monoclonal, αsm-1, Novocastra), desmin (monoclonal, D33, Dako, Glostrup, Denmark), and MyoD1 (monoclonal, 5.8A, Dako, Glostrup, Denmark). Sections were stained using Dako Envision kit (Dako, Glostrup, Denmark).

          Results

          On macroscopic examination, a nodular mass measured 17.5 × 12 × 5 cm, and showed a white-yellow to red-brown cut surface with marked necrosis (Figure 1). The distribution of adipose tissue was unclear in the tumor. Microscopic examination revealed that the tumor was composed mainly of proliferating atypical short spindle or oval cells that resembled high-grade undifferentiated sarcoma (Figure 2A). The cells showed relatively high mitotic activity with more than 5 mitoses per 10 high-power fields. Necrosis and hemorrhage were also present, and necrosis was observed in approximately 40% of the tumor. Moreover, fascicles of spindle cells containing elongated, blunt-ended nuclei and eosinophilic fibrillar cytoplasm with scattered enlarged and irregular nuclei were also detected (Figure 2B). Osteo/chondrosarcomatous differentiation and meningothelial-like whoring pattern were not observed. The presence of the WDL component was proved in additional samples from the tumor (Figure 2C).
          http://static-content.springer.com/image/art%3A10.1186%2F1746-1596-8-142/MediaObjects/13000_2013_822_Fig1_HTML.jpg
          Figure 1

          Macroscopic tumor characteristics. Nodular mass showing a white-yellow to red-brown cut surfaces with marked necrosis.

          http://static-content.springer.com/image/art%3A10.1186%2F1746-1596-8-142/MediaObjects/13000_2013_822_Fig2_HTML.jpg
          Figure 2

          Microscopic tumor characteristics. (A) Proliferation of atypical short spindle or oval cells resembling high grade undifferentiated sarcoma. (B) Proliferating spindle cells containing elongated, blunt-ended nuclei and eosinophilic fibrillar cytoplasm with scattered enlarged and irregular nuclei. (C) The presence of a WDL component, showing mature-appearing adipose tissue and fibrous bands with irregular nuclei.

          On immunohistochemical staining, the high-grade sarcomatous component was positive for MDM2 (Figure 3A) and CDK4 (Figure 3B), but not for S-100 protein, αSMA, and desmin. The spindle cell component was positive for αSMA (Figure 3C) and desmin (Figure 3D), but not for MDM2 and CDK4. Both components showed no reactivity to MyoD1. The resected margin was free, and extension from primary retroperitoneal sarcomas was not proved.
          http://static-content.springer.com/image/art%3A10.1186%2F1746-1596-8-142/MediaObjects/13000_2013_822_Fig3_HTML.jpg
          Figure 3

          Immunohistochemical analysis. (A)(B) Sarcomatous component is positive for MDM2 (A) and CDK4 (B). (C)(D) Spindle cells are positive for alpha smooth muscle actin (C) and desmin (D).

          DL with leiomyosarcomatous differentiation was diagnosed. The leiomyosarcomatous component occupied approximately 10% of the tumor. The patient was disease free after at the 2-year follow-up.

          Discussion

          Paratesticular liposarcoma is rare and has been reported in single case reports or as components of larger series. These tumors are thought to arise de novo from the adipose tissue around the spermatic cord or by malignant transformation of pre-existing lipomas [10]. In 2003, Montogomery et al studied 30 cases of paratesticular liposarcomas in men aged 41 – 87 years (mean age, 63 years) [2]. The tumors involved the spermatic cord (23 cases), testicular tunics (6 cases), or epididymis (1 case) and measured from 3 - 30 cm (mean, 11.7 cm). From the histological viewpoint, 19 cases were WDL, 10 were DL, and 1 was a myxoid/round cell liposarcoma. Among 10 DL cases, 5 were high-grade and 5 were low-grade. Leiomyosarcomatous differentiation was not described in this series. Binh et al reported 27 cases of DL with divergent myosarcomatous differentiation, including 7 cases in the paratesticular area, and leiomyosarcomatous differentiation was detected in 7 cases [9]. Henricks et al reported 155 cases of DL, including 13 cases of spermatic cord/scrotum origin, and leiomyosarcomatous differentiation was observed in 2 of the 155 cases [11]. However, the relationship between the tumor location and the histological findings was not available in both studies.

          To the best of our knowledge, only 3 well-documented cases of paratesticular liposarcoma with leiomyosarcomatous differentiation have been reported in the English literature [68]. The clinicopathological features of the reported cases, including the present case, are summarized in Table 1. In case 1, the tumor measured 7 × 3.5 × 3 cm, and was composed of ALT/WDL with well-differentiated leiomyosarcomatous component. The smooth muscle cells were arranged in well-defined bundles with no prominent nuclear pleomorphism or mitosis. Immunostaining was not performed. In case 2, the tumor measured 25 × 21 × 8 cm, and was composed of a WDL component with moderate leiomyosarcomatous differentiation. Proliferation of spindle cells, with enlarged, pleomorphic, and hyperchromatic nuclei were observed arranged in fascicles, with focal hemorrhage and necrosis in the leiomyosarcomatous area. On immunohistochemical examination, the leiomyosarcomatous components were positive for αSMA, desmin, and vimentin and negative for S-100 protein, keratin, CAM 5.2, and factor VIII-related antigen. In case 3, the tumor measured 2 cm and was composed of WDL component with leiomyosarcomatous differentiation. The smooth muscle component showed low cellularity and low nuclear grade. Immunohistochemical evaluation was not available. In the present case, the tumor was composed of a pleomorphic sarcomatous component with leiomyosarcomatous differentiation. Liposarcoma with smooth muscle differentiation was described relatively recently, and consists of 2 types: WDL having foci of mature but histologically atypical smooth muscle tissue (so-called lipoleiomyosarcoma) and DL with smooth muscle differentiation in the dedifferentiated areas (DL with heterogeneous differentiation) [8]. According to this classification, cases 1, 2, and 3 are lipoleiomyosarcoma, and the present case is DL with heterogeneous differentiation.
          Table 1

          Clinicopathological features of well-documented cases of paratesticular liposarcoma with leiomyosarcomatous differentiation

          Case

          Age (y)

          Size (cm)

          Classification of liposarcoma

          Smooth muscle component

          Recurrences

          Metastases

          Status

          Interval (mo)

          Reference

          1

          49

          7×3.5×3

          ALT/WDL

          Well-differentiated

          No

          NA

          NED

          30

          [6]

          2

          70

          25×21×8

          WDL

          Moderately differentiated

          No

          No

          NED

          72

          [7]

          3

          65

          2

          WDL

          Well-differentiated

          Yes

          No

          NED

          96

          [8]

          Current case

          77

          17.5×12.5×5

          DL

          Well-differentiated

          No

          No

          NED

          24

           

          ALT atypical lipomatous tumor, WDL well-differentiated liposarcoma, DL dedifferentiated liposarcoma, NED no evidence of disease.

          It is important to recognize the coexistence of the ALT/WDL components in order to obtain a definitive diagnosis of DL. However, if these components are not recognized, the differential diagnosis includes a variety of lesions, benign or low-grade mesenchymal tumors for low-grade differentiation, and pleomorphic sarcomas for high-grade differentiation. The general differential diagnosis of adult spindle cell tumors in this site includes primarily fibrous mesothelioma, leiomyosarcoma, malignant fibrous histiocytoma, various benign fibrous tumors, pseudotumors, and fibromatosis [2]. In addition to morphological findings, immunohistochemical study, including MDM2 and CDK4 staining, may be useful for diagnosis. The main karyotypic alteration of DL is considered to be the presence of supernumerary ring chromosomes and/or giant chromosomes composed of a chromosome 12q13-15 sequence involving MDM2 and often CDK4 genes [12]. The utility of MDM2 and CDK4 immunostaining is well documented in the diagnosis of WDL and DL [13]. However, MDM2 and CDK4 are not specific markers for liposarcoma. In the present case, the leiomyosarcomatous component showed no immunopositivity for MDM2 and CDK4; however, some leiomyosarcomas show positivity for MDM2 and CDK4 [3]. Therefore, it is important to prove the existence of the ALT/WDL component through additional sampling of the tumor.

          Clinically, the risk of local recurrence is not affected by myosarcomatous differentiation in DL, although the metastatic rate is relatively low compared to that of leiomyosarcoma. The prognosis of patients with DL with myosarcomatous differentiation is better than that of patients with leiomyosarcoma [9]. Therefore, from a clinical perspective, it is important to distinguish DL with myosarcomatous differentiation from leiomyosarcoma in an argument for conventional adjuvant chemotherapy.

          In summary, we report a rare case of paratesticular DL with with leiomyosarcomatous differentiation in a 77-year-old man. This case highlights the importance of additional sampling for obtaining a definitive diagnosis of DL, especially if the existence of an ALT/WDL component is not noted.

          Consent

          Written informed consent was obtained from the patient for publication of this case report and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.

          Declarations

          Acknowledgements

          We thank Dr. Masanori Hisaoka, Department of Pathology and Oncology, School of Medicine, University of Occupational and Environmental Health, for his thoughtful comments on the pathological diagnosis. This work was supported by a grant from the Kodama Memorial Fund for Medical Research.

          Authors’ Affiliations

          (1)
          Department of Molecular and Cellular Pathology, Kagoshima University Graduate School of Medical and Dental Sciences

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          Copyright

          © Hatanaka et al.; licensee BioMed Central Ltd. 2013

          This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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