Analysis of BRAFV600E mutation and DNA methylation improves the diagnostics of thyroid fine needle aspiration biopsies
© Zhang et al.; licensee BioMed Central Ltd. 2014
Received: 3 December 2013
Accepted: 21 February 2014
Published: 3 March 2014
Thyroid nodules with indeterminate cytological features on fine needle aspiration biopsy specimens (FNABs) have a ~20% risk of thyroid cancer. BRAF V600E mutation and DNA methylation are useful markers to distinguish malignant thyroid neoplasm from benign. The aim of this study was to determine whether combined detection of BRAF V600E mutation and methylation markers on FNABs could improve the diagnostic accuracy of thyroid cancer.
Using pyrosequencing and quantitative methylation-specific PCR (Q-MSP) methods, FNABs from 79 and 38 patients with thyroid nodules in training and test groups, respectively, were analyzed for BRAF V600E mutation and gene methylation.
BRAF V600E mutation was found in 30/42 (71.4%) and 14/20 (70%) FNABs in training and test groups, respectively. All BRAF V600E -positive samples were histologically diagnosed as papillary thyroid cancer (PTC) after thyroidectomy. As expected, BRAF mutation was not found in all benign nodules. Moreover, we demonstrated that the five genes, including CALCA, DAPK1, TIMP3, RAR-beta and RASSF1A, were aberrantly methylated in FNABs. Of them, methylation level of DAPK1 in PTCs was significantly higher than that in benign samples (P <0.0001). Conversely, methylation level of RASSF1A in PTCs was significantly lower than that in benign samples (P =0.003). Notably, compared with BRAF mutation testing alone, combined detection of BRAF mutation and methylation markers increased the diagnostic sensitivity and accuracy of PTC with excellent specificity.
Our data have demonstrated that combine analysis of BRAF mutation and DNA methylation markers on FNABs may be a useful strategy to facilitate the diagnosis of malignant thyroid neoplasm, particularly PTC.
The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/6080878071149177.
KeywordsBRAF V600E mutation DNA methylation Fine needle aspiration biopsy (FNAB) Thyroid nodules Papillary thyroid cancer (PTC)
Thyroid cancer is the most common endocrine malignancy. The current rise in the incidence of thyroid cancer is mainly from an increased incidence in papillary thyroid cancer (PTC) . The initial presentation of thyroid cancer is usually a thyroid nodule, which is palpable in approximately 5% of normal adults  and visualized by sonography in one third or more of normal adults [2, 3]. Given that the prevalence of malignancy in solitary thyroid nodules is only ~5% in adults [4, 5], the preoperative differentiation of benign from malignant thyroid nodules is imperative. Fine needle aspiration biopsy (FNAB) is the most reliable nonsurgical test for the diagnosis of thyroid cancer, with high sensitivity and specificity [6, 7]. FNAB represents the “gold standard”, and is widely used in the evaluation of thyroid nodules. However, ~20% of FNABs are diagnosed as indeterminate cytological findings that cannot distinguish cancerous from benign neoplasms [8, 9].
In addition to routine cytological examination, a number of molecular markers play an important role in the diagnosis of thyroid nodules, such as BRAF and Ras mutations, and RET/PTC rearrangements . Of them, a prominent oncogenic genetic event in PTC is BRAF V600E mutation. This mutation occurs in the majority of cases and results in the constitutively activated BRAF kinase , which plays a fundamental role in thyroid tumorigenesis through driving Ras/Raf/MEK/ERK (MAPK) signaling pathway [10, 11]. Notably, BRAF mutation has consistently been reported to be specific for PTC, whereas no benign thyroid neoplasms or normal thyroid tissues have been found to harbor this mutation. Thus, BRAF mutation has the potential to be a specific molecular marker with relatively high sensitivity for PTC diagnosis. In recent years, accumulated evidences have demonstrated that BRAF mutation detection on FNAB specimens (FNABs) enhances the diagnostic value of cytology [12–14].
Epigenetic alterations, such as promoter hypermethylation, are one of the most common molecular events in human cancers, along with genetic alterations, ultimately leading to carcinogenesis, including thyroid cancer [10, 15]. Promoter hypermethylation is an important hallmark of cancer cells and is responsible for transcriptional silencing of tumor suppressor genes during tumorigenesis [16, 17]. Aberrant DNA methylation usually occurs somatically in cancer cells and can be released into blood circulation, it may thus serve as a specific diagnostic blood test for cancer, including thyroid cancer . Hence it is not difficult to follow that detection of methylated DNA on FNABs may potentially be a useful diagnostic marker for thyroid cancer.
In this study, we detected BRAF V600E mutation and promoter methylation of a panel of potential tumor suppressor genes on FNABs using pyrosequnecing and quantitative methylation-specific PCR (Q-MSP) approaches, and determined the diagnostic value of combined analysis of these two molecular events on FNABs in the evaluation of thyroid nodules.
Patients and FNAB procedures
Clinicopathological characteristics of the patients with thyroid nodule
No. of patients in training group (%)
No. of patients in test group (%)
PTC (n =42)
Benign (n =37)
PTC (n =20)
Benign (n =18)
Tumor/nodule size (cm3)
Lymph node metastasis
For the fine needle aspiration procedure, 2 or 3 passes with 21-gauge needles were typically made to harvest material for cytological and molecular analysis. Specimens were collected in 500 μl normal saline solution in a 1.5 ml EP tube. After centrifugation, the pellet was resuspended and washed twice with normal saline solution, and final pellet was used for DNA extraction.
Genomic DNA was isolated using a protocol described previously . Briefly, the cells were incubated with 1% sodium dodecyl sulfate (SDS) and 0.5 mg/ml proteinase K at 48°C for 24–48 h. DNA was then isolated by using standard phenol/chloroform protocol, and was dissolved in distilled water and stored at −80°C until use.
Detection of BRAF V600E mutation by pyrosequencing assay
A 228-bp region of BRAF exon 15 spanning the hotspot mutation site at codon 600 was amplified by PCR using the forward and reverse primers 5′-biotin-CTT CAT AAT GCT TGC TCT GAT AGG-3′ and 5′-GGC CAA AAA TTT AAT CAG TGG AA-3′, respectively. The reaction mixture contained, in a final volume of 25 μl, ~60 ng genomic DNA, 1× PCR buffer, 1.5 mM MgCl2 , 0.2 mM of each deoxynucleotide triphosphate (dATP, dCTP, dGTP, and dTTP), 0.2 μM each primer (forward and reverse), and 0.6 U platinum DNA Taq polymerase (Invitrogen Life Technologies, Inc., MD). The PCR was performed with an initial denaturation at 95°C for 5 min, followed by 35 cycles of 95°C denaturation for 30 s, 58°C annealing for 30 s and 72°C elongation for 30 s. Quality of PCR products was determined by gel electrophoresis. The PCR product was immobilized onto streptavidin-coated sepharose beads (GE Healthcare Bio-Sciences, PA) according to the instructions of the manufacturer. The bead/DNA complex was washed and the supernatant was discarded. Sequencing primer 5′-CCACTCCATCGAGATT-3′ was added and annealed to the captured strand. Pyrosequencing assay was then performed on a PyroMark Q24 system using PyroMark Gold Q24 reagent (Qiagen).
Sodium bisulfite treatment and quantitative methylation-specific PCR (Q-MSP)
Genomic DNA was subjected to bisulfite treatment as previously described . Briefly, ~2 μg of DNA was denatured by incubation with 0.3 M NaOH at 50°C for 20 min. The denatured DNA was diluted in 500 μl of freshly prepared solution of 10 mM hydroquinone and 3 M sodium bisulfite, and incubated at 80°C for 3 h. The mixture was then purified through a Wizard DNA Clean-Up System (Promega Corp., Madison, WI) according to the instructions of the manufacturer, followed by ethanol precipitation, dry, and resuspension in 100 μl of deionized H2O.
Q-MSP primer and TaqMan probe sequences used in this study
Forward primer sequences (5′ → 3′)
Reverse primer sequence (5′ → 3′)
Non-parametric Wilcoxon’s Rank Sum test was used for comparison of the differences in methylation levels between malignant and benign nodules because of the significantly skewed distribution of the Q-MSP ratios. The association of gene methylation with BRAF mutation was determined using the Spearman’s correlation coefficient. Diagnostic threshold analyses were performed using the receiver operating characteristic (ROC) curves and the areas under the curve (AUC). All statistical analyses were performed using the SPSS statistical package (11.5, Chicago, IL, USA). P values <0.05 were considered significant.
Detection of BRAF V600E mutation on FNAB specimens
Diagnostic values of BRAF mutation and various DNA methylation markers testing on FNAB specimens for PTC detection [percent (PTCs with BRAF mutation or methylation-positive/total PTCs, sensitivity; benign nodules with BRAF mutation or methylation-negative/total benign nodules, specificity; PTCs with BRAF mutation or methylation-positive/total positive cases, PPV; benign nodules with BRAF mutation or methylation-negative/total negative nodules, NPV)]
49.12 (28/ 57)
All five genes
Quantitative analysis of DNA methylation on FNAB specimens
Correction between BRAF V600E mutation and DNA methylation
Combined detection of BRAF V600E mutation and methylation markers for improving the diagnostic accuracy of thyroid cancer
Diagnostic values of combined detection of BRAF mutation and various DNA methylation markers on FNAB specimens for PTC detection [percent (PTCs with BRAF mutation or methylation-positive/total PTCs, sensitivity; benign nodules with BRAF mutation or methylation-negative/total benign nodules, specificity; PTCs with BRAF mutation or methylation-positive/total positive cases, PPV; benign nodules with BRAF mutation or methylation-negative/total negative nodules, NPV)]
Training group (n =79)
CALCA + BRAF V600E
DAPK1 + BRAF V600E
TIMP3 + BRAF V600E
RAR-beta + BRAF V600E
RASSF1A + BRAF V600E
All five genes + BRAF V600E
Test group (n =38)
CALCA + BRAF V600E
DAPK1 + BRAF V600E
TIMP3 + BRAF V600E
RAR-beta + BRAF V600E
RASSF1A + BRAF V600E
All five genes + BRAF V600E
Thyroid nodules are among the most common endocrine complaints in the world, which sometimes represents a significant diagnostic challenge in differentiating malignant from benign lesions. Currently, morphological diagnosis methods, such as immunohistochemistry and cytology, play an important role in differentiating malignant from benign nodules. For instance, depending on the histomorphologic features of routine hematoxylin and eosin (H&E), cytology of FNABs often provides a definitive diagnosis and sound therapeutic guidance. However, a substantial number of cases cannot be diagnosed by this technique and many cases generate indeterminate results . Nowadays, the role of molecular markers in cancer diagnosis and treatment has been established, including thyroid cancer [25, 26]. For example, galectin-3, fibronectin-1, CK-19, CK-903, CITED1, Ret oncoprotein, TG, Ki67, anti-MAP kinase, p16, and the mesothelial cell surface protein HBME-1 have been used as preoperative diagnostic markers [27–32]. Unfortunately, none of them are specific for thyroid malignancies.
In recent years, testing for the BRAF V600E mutation has been extensively used to improve the diagnostic accuracy of thyroid malignancy in nodules, because of the high specificity of this mutation for PTC [12–14, 19, 25, 26, 33]. BRAF V600E mutation, which is the most common oncogenic genetic event found in thyroid cancer, particularly in PTC, results in constitutive and oncogenic activation of BRAF kinase in the MAPK signaling pathway . Through activating MAPK pathway, the BRAF mutation plays a fundamental role in the tumorigenesis of PTC and predicts its poor clinical outcomes [10, 11]. In this study, we tested the role of BRAF mutation in the diagnosis of malignant thyroid nodules. The data showed that the prevalence of BRAF mutation was more than 70%, and was highly specific for PTCs, as supported by our data that all BRAF V600E -positive cases were histologically diagnosed as PTC after thyroidectomy. These observations suggest that molecular testing for BRAF mutation has an important impact on the diagnosis of thyroid malignant nodules, particularly PTC, as well as on decisions concerning the extent of surgery, particularly in the cases with indeterminate fine-needle aspiration cytology. Generally, some patients with indeterminate cytological findings undergo total thyroidectomy, whereas others choose to receive hemithyroidectomy. The patients in the latter group who prove to have thyroid cancer are usually advised to undergo completion thyroidectomy. Thus, for thyroid nodule patients, particularly the patients with indeterminate cytological findings, preoperative evaluation of a specific molecular marker, such as BRAF mutation, would be greatly helpful. In addition, accumulated evidences have demonstrated that the patients with BRAF mutation show frequent recurrence and resistance to radioactive iodine therapy, BRAF mutation may also be used as a prognostic factor [34, 35]. BRAF mutation testing would thus be a very informative and useful tool in the management of thyroid nodules and cancers.
In addition to genetic factors, epigenetic events, such as aberrant gene methylation, play a critical role in thyroid tumorigenesis . DNA methylation is one of major mechanisms of inactivation of tumor-related genes, particularly tumor suppressor genes, in cancer cells. The advantages of gene methylation as a molecular marker for the detection and diagnosis cancer in biopsy specimens and non-invasive body fluids, such as serum, has led to many studies of application in human cancers, including thyroid cancer . Similarly, we hypothesize that there is potential value of gene methylation testing on FNABs as diagnostic markers to distinguish malignant from benign nodules. There are several reasons to support our hypothesis. First, DNA methylation commonly occurs in thyroid cancer . Second, in generate, the amount of DNA from cancer cells in FNABs is much greater than in serum samples. Third, DNA, unlike mRNA, is a stable molecule, offering the promise of greater test stability. Fourth, DNA methylation can be quantitatively analyzed by Q-MSP assay used in this study, which has become a well-established and widely available technique [18, 20, 22, 24]. In this study, we have confirmed the measurability of FNAB methylation markers and preliminarily defined the specificity and sensitivity of a panel of methylation markers for evaluating thyroid nodules. Using these five genes, CALCA, RAR-beta, DAPK1, TIMP3, and RASSF1A for differential diagnosis of thyroid nodules with appropriate cut-off values, we were able to identify positive gene methylation in 33.33-88.10% of malignant nodules, but the diagnostic specificities were all low, ranging from 75.68 to 83.78%. Although DAPK1 and RASSF1A genes exhibited significantly different levels of DNA methylation between malignant and benign nodules, their diagnostic accuracy was still poor. These findings suggest that quantitative detection of these methylation markers on FNABs has serious limitations in the diagnosis of thyroid cancer.
Notably, the analysis of BRAF mutation confirmed the previously reported inverse association with RASSF1A methylation [22, 24, 36, 37]. In addition, our data showed a positive correlation between DAPK1 methylation and BRAF mutation. To our knowledge this is the first evidence indicating a relationship between these two molecular alterations in thyroid cancer. Given that both of BRAF mutation and gene methylation play a key role in thyroid carcinogenesis and there is certain relationship between them, we presume that combined detection of these two molecular events on FNABs may improve the diagnostic accuracy of thyroid nodules. To this end, we performed BRAF mutation and DNA methylation assays in training and test groups, respectively, and demonstrated that, as compared with BRAF mutation and DNA methylation testing each alone, combined analysis of these two molecular events increased the diagnostic sensitivity and accuracy, with excellent specificity (94.44-100%).
In summary, combined detection of BRAF mutation and methylation markers on FNABs may be a useful strategy in evaluation of thyroid nodules with indeterminate cytological findings. Importantly, such a combined analysis will make preoperative risk and prognostic evaluation of malignant thyroid neoplasm more accurate and effective.
This work was supported by the National Natural Science Foundation of China (No. 81171969 and 81372217), the Fundamental Research Funds for the Central Universities, the Ph. D. Programs Foundation of Ministry of Education of China (No. 2012021110058), and the Program for New Century Excellent Talents in University (No. NCET-10-0674).
- Davies L, Welch HG: Increasing incidence of thyroid cancer in the United States, 1973–2002. JAMA. 2006, 295: 2164-2167. 10.1001/jama.295.18.2164.PubMedView ArticleGoogle Scholar
- Xing M, Tufano RP, Tufaro AP, Basaria S, Ewertz M, Rosenbaum E, Byrne PJ, Wang J, Sidransky D, Ladenson PW: Detection of BRAF mutation on fine needle aspiration biopsy specimens: a new diagnostic tool for papillary thyroid cancer. J Clin Endocrinol Metab. 2004, 89: 2867-2872. 10.1210/jc.2003-032050.PubMedView ArticleGoogle Scholar
- Brander A, Viikinkoski P, Nickels J, Kivisaari L: Thyroid gland: US screening in a random adult population. Radiology. 1991, 181: 683-687.PubMedView ArticleGoogle Scholar
- Bruneton JN, Balu-Maestro C, Marcy PY, Melia P, Mourou MY: Very high frequency (13 MHz) ultrasonographic examination of the normal neck: detection of normal lymph nodes and thyroid nodules. J Ultrasound Med. 1994, 13: 87-90.PubMedGoogle Scholar
- Werk EE, Vernon BM, Gonzalez JJ, Ungaro PC, McCoy RC: Cancer in thyroid nodules. A community hospital survey. Arch Intern Med. 1984, 144: 474-476. 10.1001/archinte.1984.00350150058018.PubMedView ArticleGoogle Scholar
- Belfiore A, Giuffrida D, la Rosa GL, Ippolito O, Russo G, Fiumara A, Vigneri R, Filetti S: High frequency of cancer in cold thyroid nodules occurring at young age. Acta Endocrinol (Copenh). 1989, 121: 197-202.Google Scholar
- Ravetto C, Colombo L, Dottorini ME: Usefulness of fine-needle aspiration in the diagnosis of thyroid carcinoma: a retrospective study in 37,895 patients. Cancer. 2000, 90: 357-363. 10.1002/1097-0142(20001225)90:6<357::AID-CNCR6>3.0.CO;2-4.PubMedView ArticleGoogle Scholar
- Werga P, Wallin G, Skoog L, Hamberger B: Expanding role of fine-needle aspiration cytology in thyroid diagnosis and management. World J Surg. 2000, 24: 907-912. 10.1007/s002680010163.PubMedView ArticleGoogle Scholar
- Gharib H, Goellner JR, Zinsmeister AR, Grant CS, van Heerden JA: Fine-needle aspiration biopsy of the thyroid. The problem of suspicious cytologic findings. Ann Intern Med. 1984, 101: 25-28. 10.7326/0003-4819-101-1-25.PubMedView ArticleGoogle Scholar
- Xing M: Molecular pathogenesis and mechanisms of thyroid cancer. Nat Rev Cancer. 2013, 13: 184-199. 10.1038/nrc3431.PubMedPubMed CentralView ArticleGoogle Scholar
- Xing M: BRAF mutation in papillary thyroid cancer: pathogenic role, molecular bases, and clinical implications. Endocr Rev. 2007, 28: 742-762. 10.1210/er.2007-0007.PubMedView ArticleGoogle Scholar
- Chung KW, Yang SK, Lee GK, Kim EY, Kwon S, Lee SH, Park do J, Lee HS, Cho BY, Lee ES, Kim SW: Detection of BRAFV600E mutation on fine needle aspiration specimens of thyroid nodule refines cyto-pathology diagnosis, especially in BRAF600E mutation-prevalent area. Clin Endocrinol (Oxf). 2006, 65: 660-666. 10.1111/j.1365-2265.2006.02646.x.View ArticleGoogle Scholar
- Zatelli MC, Trasforini G, Leoni S, Frigato G, Buratto M, Tagliati F, Rossi R, Cavazzini L, Roti E, degli Uberti EC: BRAF V600E mutation analysis increases diagnostic accuracy for papillary thyroid carcinoma in fine-needle aspiration biopsies. Eur J Endocrinol. 2009, 161: 467-473. 10.1530/EJE-09-0353.PubMedView ArticleGoogle Scholar
- Nikiforov YE, Steward DL, Robinson-Smith TM, Haugen BR, Klopper JP, Zhu Z, Fagin JA, Falciglia M, Weber K, Nikiforova MN: Molecular testing for mutations in improving the fine-needle aspiration diagnosis of thyroid nodules. J Clin Endocrinol Metab. 2009, 94: 2092-2098. 10.1210/jc.2009-0247.PubMedView ArticleGoogle Scholar
- Xing M: Gene methylation in thyroid tumorigenesis. Endocrinology. 2007, 148: 948-953. 10.1210/en.2006-0927.PubMedView ArticleGoogle Scholar
- Herman JG, Baylin SB: Gene silencing in cancer in association with promoter hypermethylation. N Engl J Med. 2003, 349: 2042-2054. 10.1056/NEJMra023075.PubMedView ArticleGoogle Scholar
- Jones PA, Baylin SB: The fundamental role of epigenetic events in cancer. Nat Rev Genet. 2002, 3: 415-428.PubMedView ArticleGoogle Scholar
- Hu S, Ewertz M, Tufano RP, Brait M, Carvalho AL, Liu D, Tufaro AP, Basaria S, Cooper DS, Sidransky D, Ladenson PW, Xing M: Detection of serum deoxyribonucleic acid methylation markers: a novel diagnostic tool for thyroid cancer. J Clin Endocrinol Metab. 2006, 91: 98-104. 10.1210/jc.2005-1810.PubMedView ArticleGoogle Scholar
- Xing M, Usadel H, Cohen Y, Tokumaru Y, Guo Z, Westra WB, Tong BC, Tallini G, Udelsman R, Califano JA, Ladenson PW, Sidransky D: Methylation of the thyroid stimulating hormone receptor gene in epithelial thyroid tumors: a marker of malignancy and a cause of gene silencing. Cancer Res. 2003, 63: 2316-2321.PubMedGoogle Scholar
- Ji M, Zhang Y, Shi B, Hou P: Association of promoter methylation with histologic type and pleural indentation in non-small cell lung cancer (NSCLC). Diagn Pathol. 2011, 6: 48-10.1186/1746-1596-6-48.PubMedPubMed CentralView ArticleGoogle Scholar
- Hu S, Liu D, Tufano RP, Carson KA, Rosenbaum E, Cohen Y, Holt EH, Kiseljak-Vassiliades K, Rhoden KJ, Tolaney S, Condouris S, Tallini G, Westra WH, Umbricht CB, Zeiger MA, Califano JA, Vasko V, Xing M: Association of aberrant methylation of tumor suppressor genes with tumor aggressiveness and BRAF mutation in papillary thyroid cancer. Int J Cancer. 2006, 119: 2322-2329. 10.1002/ijc.22110.PubMedView ArticleGoogle Scholar
- Hoque MO, Rosenbaum E, Westra WH, Xing M, Ladenson P, Zeiger MA, Sidransky D, Umbricht CB: Quantitative assessment of promoter methylation profiles in thyroid neoplasms. J Clin Endocrinol Metab. 2005, 90: 4011-4018. 10.1210/jc.2005-0313.PubMedView ArticleGoogle Scholar
- Hou P, Liu D, Xing M: Genome-wide alterations in gene methylation by the BRAF V600E mutation in papillary thyroid cancer cells. Endocr Relat Cancer. 2011, 18: 687-697. 10.1530/ERC-11-0212.PubMedPubMed CentralView ArticleGoogle Scholar
- Brait M, Loyo M, Rosenbaum E, Ostrow KL, Markova A, Papagerakis S, Zahurak M, Goodman SM, Zeiger M, Sidransky D, Umbricht CB, Hoque MO: Correlation between BRAF mutation and promoter methylation of TIMP3, RARβ2 and RASSF1A in thyroid cancer. Epigenetics. 2012, 7: 710-719. 10.4161/epi.20524.PubMedPubMed CentralView ArticleGoogle Scholar
- Nikiforova MN, Nikiforov YE: Molecular diagnostics and predictors in thyroid cancer. Thyroid. 2009, 19: 1351-1361. 10.1089/thy.2009.0240.PubMedView ArticleGoogle Scholar
- Handkiewicz-Junak D, Czarniecka A, Jarzab B: Molecular prognostic markers in papillary and follicular thyroid cancer: Current status and future directions. Mol Cell Endocrinol. 2010, 322: 8-28. 10.1016/j.mce.2010.01.007.PubMedView ArticleGoogle Scholar
- Barroeta JE, Baloch ZW, Lal P, Pasha TL, Zhang PJ, LiVolsi VA: Diagnostic value of differential expression of CK19, Galectin-3, HBME-1, ERK, RET, and p16 in benign and malignant follicular-derived lesions of the thyroid: an immunohistochemical tissue microarray analysis. Endocr Pathol. 2006, 17: 225-234. 10.1385/EP:17:3:225.PubMedView ArticleGoogle Scholar
- Bojunga J, Zeuzem S: Molecular detection of thyroid cancer: an update. Clin Endocrinol (Oxf). 2004, 61: 523-530. 10.1111/j.1365-2265.2004.02131.x.View ArticleGoogle Scholar
- Niedziela M, Maceluch J, Korman E: Galectin-3 is not an universal marker of malignancy in thyroid nodular disease in children and adolescents. J Clin Endocrinol Metab. 2002, 87: 4411-4415. 10.1210/jc.2002-020387.PubMedView ArticleGoogle Scholar
- Prasad ML, Pellegata NS, Huang Y, Nagaraja HN, de la Chapelle A, Kloos RT: Galectin-3, fibronectin-1, CITED-1, HBME1 and cytokeratin-19 immunohistochemistry is useful for the differential diagnosis of thyroid tumors. Mod Pathol. 2005, 18: 48-57. 10.1038/modpathol.3800235.PubMedView ArticleGoogle Scholar
- Saleh HA, Jin B, Barnwell J, Alzohaili O: Utility of immunohistochemical markers in differentiating benign from malignant follicular derived thyroid nodules. Diagn Pathol. 2010, 5: 9-10.1186/1746-1596-5-9.PubMedPubMed CentralView ArticleGoogle Scholar
- Song Q, Wang D, Lou Y, Li C, Fang C, He X, Li J: Diagnostic significance of CK19, TG, Ki67 and galectin-3 expression for papillary thyroid carcinoma in the northeastern region of China. Diagn Pathol. 2011, 6: 126-10.1186/1746-1596-6-126.PubMedPubMed CentralView ArticleGoogle Scholar
- Kang S, Ahn S, Lee SM, Jeong JY, Sung JY, Oh YL, Kim KM: Shifted termination assay (STA) fragment analysis to detect BRAF V600 mutations in papillary thyroid carcinomas. Diagn Pathol. 2013, 8: 121-10.1186/1746-1596-8-121.PubMedPubMed CentralView ArticleGoogle Scholar
- Xing M: Prognostic utility of BRAF mutation in papillary thyroid cancer. Mol Cell Endocrinol. 2010, 321: 86-93. 10.1016/j.mce.2009.10.012.PubMedPubMed CentralView ArticleGoogle Scholar
- Melck AL, Yip L, Carty SE: The utility of BRAF testing in the management of papillary thyroid cancer. Oncologist. 2010, 15: 1285-1293. 10.1634/theoncologist.2010-0156.PubMedPubMed CentralView ArticleGoogle Scholar
- Nakamura N, Carney JA, Jin L, Kajita S, Pallares J, Zhang H, Qian X, Sebo TJ, Erickson LA, Lloyd RV: RASSF1A and NORE1A methylation and BRAFV600E mutations in thyroid tumors. Lab Invest. 2005, 85: 1065-1075. 10.1038/labinvest.3700306.PubMedView ArticleGoogle Scholar
- Xing M, Cohen Y, Mambo E, Tallini G, Udelsman R, Ladenson PW, Sidransky D: Early occurrence of RASSF1A hypermethylation and its mutual exclusion with BRAF mutation in thyroid tumorigenesis. Cancer Res. 2004, 64: 1664-1668. 10.1158/0008-5472.CAN-03-3242.PubMedView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.