Table 2 Image volume in relation to objective magnification and optical resolution
Objective magnification | 4* | 10* | 20* | 40* |
|---|---|---|---|---|
Numerical aperture | 0.2 | 0.45 | 0.5 | 0.75 |
Optical resolution (μm) | 1.7 | 0.75 | 0.67 | 0.45 |
Pixel number* | 11765*14704 | 26667*33333 | 29851*37313 | 44444*55556 |
Image size | 2.08 GB | 11 GB | 13 GB | 30 GB |
Object size | 1 – 4 MB | 1 MB | 5 MB | 12 MB |
Sample size** | 8 – 32 MB | 8 MB | 40 MB | 120 MB |
Nuclei | - | - | * | * |
Cells | - | (*) | * | * |
Vessels | * | * | (*) | - |
Sample Number*** | 6 – 25 | 1375 | 325 | 25 |