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Table 3 UCCC scoring and interpretation of different types of EGFR gene amplification in NSCLC specimens.

From: Stratification of non-small cell lung cancer patients for therapy with epidermal growth factor receptor inhibitors: the EGFR fluorescence in situ hybridization assay

Pattern Description Scoring Criteria Number of Cells to Score Expected Ratio Gene to Chromosome FISH result*
Large, loose clusters of red (EGFR) signals Count signals thoroughly and calculate the FISH indexes 30 >2 GA
Small, loose clusters of red (EGFR) signals Count signals thoroughly and calculate the FISH indexes 30 >2 or ~1 if high level of aneusomy 7 is present GA if ratio >2 GA if clusters are present in >10% cells, independent of ratio
Large, loose co-localized clusters of red (EGFR) and green (CEP 7) signals Count signals thoroughly, account for the innumerable signals using the symbol greater than (>) in front of the final counting 30 ~1 GA if clusters are present in >10% cells
Tightly packed, innumerable cluster of red (EGFR) signals or atypically large red (EGFR) signal, consistently bigger than the green (CEP 7) signal in the tumor cells but smaller in the adjacent stromal and reactive cells Count signals in at least 50 cells and identify each cell displaying this specific feature in the analysis sheet ≥ 50 ~1 GA if tight clusters or atypical red signal is present in >10% cells
EGFR as double minutes Count signals thoroughly and calculate the FISH indexes 30 >2 GA
Very high number of balanced red (EGFR) and green (CEP 7) signals Count signals in at least 50 cells ≥50 ~1 GA if >10% cells have ≥ 15 red (EGFR) signals
  1. * GA = EGFR gene amplification