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Table 1 Antibodies used in the study

From: Smooth muscle actin and s100p on non germinal centre diffuse large B cell lymphoma are adverse prognostic factors: pilot study

Antibodies

Source

Clone/Antibody

HIER

Dilution

Incubation

Staining interpretation

S100p

DakoCytomation

Rabbit polyclonal

1

1: 400

RT

Nuclear -/+cytoplasmic

SMA

DakoCytomation

1A4/monoclonal

2

1:50

RT

Nuclear -/+cytoplasmic

CD20

DakoCytomation

L26/monoclonal

2

1:200

RT

Membranous

CD79a

DakoCytomation

JCB117/monoclonal

2

1:50

RT

Membranous

CD10

Novocastra

NCL-CD10-270/monoclonal

1

1:20

RT

Membranous

Bcl-6

DakoCytomation

BG-B6p/monoclonal

1

1:20

RT

Nuclear

Mum-1

Santa Cruz

goat polyclonal

2

1:50

37°C

Nuclear, nucleolar

Bcl-2

DakoCytomation

124/monoclonal

2

1:50

RT

Membranous

Ki-67

DakoCytomation

MIB-1/monoclonal

1

1:75

RT

Nuclear, nucleolar

  1. 1-Tris-EDTA pH9.0
  2. 2-Citrate buffer pH6.0
  3. RT: room temperature