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Figure 4 | Diagnostic Pathology

Figure 4

From: Development of automated brightfield double In Situ hybridization (BDISH) application for HER2 g ene and chromosome 17 centromere (CEN 17) for breast carcinomas and an assay performance comparison to manual dual color HER2 fluorescence In Situ hybridization (FISH)

Figure 4

The heterogeneity of breast tumor cell populations. The heterogeneity of breast cancer cells was demonstrated with brightfield double in situ hybridization (BDISH) stained tissue sections. In general, there were 2 types of the tumor cell heterogeneity: 1) variegated tumor populations of, as an example, non-amplified (blue asterisk) and amplified (yellow asterisk) HER2 gene cells in the same area (A) and 2) segregated tumor populations of, as an example, discrete (B) and clustered (C) HER2 gene cells in different areas. The tumor cell heterogeneity with different appearance of HER2 and CEN 17 is also seen among the non-large clustered HER2 cells (B). 100×.

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