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Table 3 Performance of brightfield double in situ hybridization (BDISH) with clinical samples based on the ASCO/CAP method without 4 FISH equivocal cases

From: Development of automated brightfield double In Situ hybridization (BDISH) application for HER2 g ene and chromosome 17 centromere (CEN 17) for breast carcinomas and an assay performance comparison to manual dual color HER2 fluorescence In Situ hybridization (FISH)

  

FISH

Total

  

Positive

Negative

 

BDISH

Positive

25

0

25

 

Negative

0

63

63

 

Total

25

63

88

  1. Frequency missing = 2
  2. Sensitivity 100%
  3. Specificity 100%
  4. Concordance 100%
  5. Kappa 1.0000