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Table 2 Explanations: H & E: hematoxilin – eosin; DAB: diaminobencidine; AP: Alcalic phosphatase; DAPI & Texas Red & FITC: tri-colour fluorescent stain

From: How to measure image quality in tissue-based diagnosis (diagnostic surgical pathology)

Stain Number of thresholds Area covered first threshold Area covered 2nd threshold
H & E 3 54% 10%
DAB 3 63% 12%
AP 3 45% 11%
(DAPI & TEXAS RED & FITC 2 35% 5%