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Table 2 Explanations: H & E: hematoxilin – eosin; DAB: diaminobencidine; AP: Alcalic phosphatase; DAPI & Texas Red & FITC: tri-colour fluorescent stain

From: How to measure image quality in tissue-based diagnosis (diagnostic surgical pathology)

Stain

Number of thresholds

Area covered first threshold

Area covered 2nd threshold

H & E

3

54%

10%

DAB

3

63%

12%

AP

3

45%

11%

(DAPI & TEXAS RED & FITC

2

35%

5%