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Table 1 List of Antibodies for Immunohistochemical Studies

From: Giant cell tumor-like lesion of the urinary bladder: a report of two cases and literature review; giant cell tumor or undifferentiated carcinoma?

Antibody Clone Dilution Antigen retrieval method Source
Vimentin V9 1:150 Citrate buffer (20 min) Neomarkers, Fremont, CA, USA
Epithelial membrane antigen (EMA) GP1.1 1:150 Citrate buffer (20 min) DBS, Pleasanton, CA
Pan-cytokeratin AE1/AE3 1:50 Citrate buffer (20 min) Neomarkers
Cytokeratin 7 OV-TL 12/30 1:200 Tripsin (10 min at 37°C) Neomarkers
Cytokeratin 20 Ks20.8 1:100 Tripsin (10 min at 37°C) Neomarkers
Desmin D33 1:100 Citrate buffer (20 min) Neomarkers
α-Actin (SMA) 1A4 1:200 Citrate buffer (20 min) Neomarkers
S-100 protein 4C4.9 1:150 Citrate buffer (20 min) Neomarkers
Leukocyte common antigen (LCA) Cocktail PanLCA 1:200 Citrate buffer (20 min) Neomarkers
CD68 PG-M1 1:100 Citrate buffer (20 min) Neomarkers
Ki-67 SP6 1:200 Citrate buffer (20 min) Neomarkers
P53 DO7 Prediluted Citrate buffer (20 min) Dako, Carpenteria, CA, USA
TRAP 26E5 Prediluted Citrate buffer (20 min) Neomarkers