Table 1 List of Antibodies for Immunohistochemical Studies
Antibody | Clone | Dilution | Antigen retrieval method | Source |
|---|---|---|---|---|
Vimentin | V9 | 1:150 | Citrate buffer (20 min) | Neomarkers, Fremont, CA, USA |
Epithelial membrane antigen (EMA) | GP1.1 | 1:150 | Citrate buffer (20 min) | DBS, Pleasanton, CA |
Pan-cytokeratin | AE1/AE3 | 1:50 | Citrate buffer (20 min) | Neomarkers |
Cytokeratin 7 | OV-TL 12/30 | 1:200 | Tripsin (10 min at 37°C) | Neomarkers |
Cytokeratin 20 | Ks20.8 | 1:100 | Tripsin (10 min at 37°C) | Neomarkers |
Desmin | D33 | 1:100 | Citrate buffer (20 min) | Neomarkers |
α-Actin (SMA) | 1A4 | 1:200 | Citrate buffer (20 min) | Neomarkers |
S-100 protein | 4C4.9 | 1:150 | Citrate buffer (20 min) | Neomarkers |
Leukocyte common antigen (LCA) | Cocktail PanLCA | 1:200 | Citrate buffer (20 min) | Neomarkers |
CD68 | PG-M1 | 1:100 | Citrate buffer (20 min) | Neomarkers |
Ki-67 | SP6 | 1:200 | Citrate buffer (20 min) | Neomarkers |
P53 | DO7 | Prediluted | Citrate buffer (20 min) | Dako, Carpenteria, CA, USA |
TRAP | 26E5 | Prediluted | Citrate buffer (20 min) | Neomarkers |