Table 1 Comparison of GTC-based versus SGC-based techniques
GTC | SGC |
|---|---|
works best either with high (300 mg) or very low (20 mg) amount of tissue, because of phenol-chloroform follows by ethanol precipitation steps and uses of denaturants and RNase inhibitors | works best with low (<100 mg) amount of tissue, because filters may clog resulting in low yield of total RNA |
Several chemical treatment steps in protocol may affect RNA quality | Limited steps in protocol may not have influences on RNA intactness |
Complex protocol, takes 4 hrs | Simple protocol, takes only 20 min |
chemically based aqueous-phase procedure, increased risk of cross contamination | No phase separation step present, low risk of cross contamination |
Use of hazardous chemicals like phenol and chloroform requires specific safety rules in the laboratory | Absolutely safe while handling. |
less expensive than SGC method | More expensive than GTC method |