Table 2 Primer details: Sequence, annealing temperature (AT), and amplicon size (AS) of target sequence
Target gene | Sequence (FP; 5' to 3') | AT [°C] | AS [bp] |
|---|---|---|---|
Sequence (RP; 5' to 3') | |||
GAPDH | AATGGTAAGGTCGGTGTGTGAA | 60 | 262 |
GAAGATGGTGATGGGCTTCC | |||
GAPDH-3' UTR | ACAGGGTGGTGGACCTCATG | 60 | 102 |
GTTGGGATAGGGCCTCTCTTG | |||
PBGD | TGCACGATCCTGAAACTCTG | 60 | 163 |
28S | TCATCAGACCCCAGAAAAGG | 60 | 102 |
GGCCTCACTAAACCATCCAA |