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Table 1 erb B antibodies used

From: Overexpression of c-erb B2 is a negative prognostic factor in anaplastic astrocytomas

Antibody Source Type Clone Reactant Positive control Dilution Incubation time and temperature
NCL-EGFR-384 Novocastra, Newcastle-upon-Tyne, UK Monoclonal EGFR.25 EGFR (c-erb B1 protein) (internal domain) Human skin 1:100 60 min. at room temperature
NCL-EGFR Novocastra Monoclonal EGFR.113 EGFR (c-erb B1 protein) (external domain) Human skin 1:10 60 min. at room temperature
c-erb B-2 Novocastra Monoclonal CB11 c-erb B2 protein (internal domain) SKBR-3 breast cancer cell line 1:40 60 min. at room temperature
c-erb B-2 Immunotech, Marseille, France Monoclonal 3B5 c-erb B2 protein (internal domain) SKBR-3 breast cancer cell line Ready to use 60 min. at room temperature
c-erb B-2 Novocastra Monoclonal 5A2 c-erb B2 protein (internal domain) SKBR-3 breast cancer cell line 1:50 60 min. at room temperature
NCL-c-erb B-3 Novocastra Monoclonal RTJ1 c-erb B-3 protein Normal kidney 1:10 Over night at 4°C
c-erb B-4/HER4 NeoMarkers (Fremont, CA, USA) Monoclonal HFR-1 c-erb B-4 protein Breast carcinoma 1:10 Over night at 4°C