Table 2 KRAS Mutations Reported by Mutation Analysis Method
Sample | Direct Sequencing (Amgen) | Allele-Specific PCR (HistoGeneX/DxS) | Direct Sequencing (Gentris) | Allele-Specific Hybridization (Invitek) | Allele-Specific PCR Extensions (Genzyme) | Picotiter Plate Sequencing (Agencourt)* |
|---|---|---|---|---|---|---|
Samples for which a KRAS sequence determination was made, n | 40 | 40 | 32 | 27 | 35 | 32 |
Total WT, n | 20 | 21 | 18 | 3 | 15 | 15 |
1 | G13D | G13D | G13D | G13D | G13D | G13D (4219/7836; 0.54)** |
2 | WT | WT | WT | Inconclusive† | WT | WT (8880)** |
3 | G13D | G13D | 12WT, no call 13† | G13D | G13D | G13D (1679/8282; 0.2) |
4 | WT | WT | WT | Inconclusive† | WT | WT (8371) |
5 | WT | WT | WT | G13D | WT | Not tested |
6 | G12A | G12A | G12A | WT | G12A | No result† |
7 | G12S, G13D | G12S | G12S, no call 13† | Inconclusive† | Failed† | G12S (53/825; 0.06) |
8 | G12V, G13D | G12V | G12V | G12S, G12V | G12V | G12V (244/1289; 0.19), G13D (53/1289; 0.04) |
9 | WT | WT | WT | Not analyzed‡ | Failed† | G12V (43/106; 0.41) |
10 | WT | WT | 12WT, no call 13† | WT | Failed† | WT (18) |
11 | WT | WT | Mixed sequence§ | Not analyzed‡ | Failed† | No result† |
12 | G12V | G12V | G12V | G12D, G13D | G12V | G12V (2443/4707; 0.52) |
13 | WT | WT | WT | G13D | WT | WT (9223) |
14 | WT | WT | WT | Inconclusive† | WT | WT (9921) |
15 | G12D | G12D | G12D | G12D, G13D | G12D | G12D (4178/8006; 0.52) |
16 | WT | WT | WT | Inconclusive† | WT | WT (7001) |
17 | G13D | G13D | No call 12 or 13† | G13D | G13D | Not tested|| |
18 | G12A | G12A | G12A | G12A, G13D | G12A | Not tested|| |
19 | WT | WT | WT | G13D | WT | WT (8891) |
20 | G12D | G12D | G12D | G12D | G12D | G12D (2513/4304; 0.58) |
21 | G13D | G12D | WT | G12D, G13D | G12D, G13D | G12D (761/6260; 0.12), G13D (1639/6260; 0.26) |
22 | WT | WT | WT | Inconclusive† | WT | WT (2908) |
23 | G12D | G12D | WT | Inconclusive† | G12D | G12D (2115/7262; 0.29) |
24 | WT | WT | No amplification¶ | WT | Failed† | WT (71) |
25 | WT | WT | WT | G13D | WT | WT (2477) |
26 | WT | WT | WT | Inconclusive† | WT | WT (5887) |
27 | G12V | G12V | G12V | G12V | G12V | G12V (1812/6404; 0.28) |
28 | WT | WT | WT | Inconclusive† | WT | WT (8696) |
29 | WT | WT | G12V | G13D | WT | WT (3876) |
30 | G13D | G13D | G13D | G13D | G13D | G13D (4697/9754; 0.48) |
31 | WT | WT | No amplification | G12D, G13D | G13D | No result† |
32 | WT | WT | WT | G13D | WT | WT (5048) |
33 | G12V | G12V | WT | G12V, G13D | G12V | G12V (505/1042; 0.48) |
34 | WT | WT | WT | G13D | WT | WT (6329) |
35 | G13D | G13D | G13D | G13D | G13D | G13D (2578/7158; 0.36) |
36 | G12V, G13D | G12V | G12V | Inconclusive† | G12V | Not tested|| |
37 | G12D | G12D | G12D | G12D, G13D | G12D | G12D (14/61; 0.23) |
38 | G13D | WT | 12WT, no call 13† | G13D | G13D | G13D (1539/5423; 0.28) |
39 | WT | WT | WT | G13D | WT | Not tested|| |
40 | G12D | G12D | G12D | Inconclusive† | G12D | G12D (1884/4565; 0.41) |