Figure 3

Adhesion assay of stable transfectant cells with an empty vector (KYSE 170 mock) compared to a siRNA vector against fascin (KYSE 170F4). (A) Total number of cells, number of floating dead cells and number of adherent living cells for stable transfectant cells with an empty vector (KYSE 170 mock) as compared to a siRNA vector against fascin (KYSE 170F4). Left, the total number of cells (× 10⁴ cells/dish). Middle, the number of floating dead cells (× 10³ cells/ml). Right, the number of adherent living cells (× 10³ cells/ml). (B) Photographs of ESCC 24 hours after seeding. (a') Adherent stable transfectant cells with an empty vector (KYSE 170 mock). (b') Floating stable transfectant cells with an empty vector (KYSE 170 mock). (c') Adherent stable transfectant cells with a siRNA vector against fascin (KYSE 170F4). (d') Floating stable transfectant cells with a siRNA vector against fascin (KYSE 170F4). (C) Effect of fascin knockdown and the effect of caspase dependency in cell growth. The cells were treated with 200 μmol/L of the general caspase inhibitor Z-VAD-FMK (+). (D) Control, without the general caspase inhibitor Z-VAD-FMK (-).