Table 1 Immunohistochemical markers used for malignant mesothelioma diagnosis in this study
From: Re-evaluation of histological diagnoses of malignant mesothelioma by immunohistochemistry
IHC marker | Antigen type | Clone | Producer | Code No. | Dilution |
|---|---|---|---|---|---|
Positive markers | |||||
Calretinin | Calcium-binding protein | polyclonal | Zymed | 18-0211 | 1: 1,500 |
EMA | HMFG protein | E-29 | Dako | M0613 | 1: 750 |
Thrombomodulin | endothelial cell transmembrane glycoprotein | 1009 | Dako | M0617 | 1: 50 |
HBME-1 | mesothelial cell membrane protein | HBME-1 | Dako | M3505 | 1: 50 |
Mesothelin | cell surface glycoprotein | 5B2 | Novocastra | NCL-L-MESO | 1: 10 |
Podoplanin | transmembrane mucoprotein | n.s., monoclonal | Angiobio | 11-003 | 1: 50 |
CK5/6 | IMF | D5/16 B4 | Dako | M7237 | 1: 80 |
CK7 | IMF | OV-TL12/30 | Dako | M7018 | 1: 1,000 |
CK AE1/AE3 | IMF (pan-CK cocktail, subfamilies A and B) | AE1 and AE3 | Dako | N1590 | 1: 75 |
CK KL1 | IMF (pan-CK cocktail) | KL1 | Serotec | MCA144H | 1: 50 |
Negative markers | |||||
monoclonal CEA | Gold 1 epitope | II-7 | Dako | M7072 | 1: 200 |
polyclonal CEA | CEA and CEA-like proteins | polyclonal | Dako | A0115 | 1: 13,000 |
Ber-Ep4 | transmembrane glycoprotein | Ber-Ep4 | Dako | M 0804 | 1: 200 |
CD15 | Lewis X carbohydrate antigen | C3D-1 | Dako | M0733 | 1: 25 |
Sialyl-TN | Sialosyl-Tn1 glycoprotein | HB-STn1 | Dako | M0899 | 1: 100 |
TTF-1 | nuclear transcription factor | 8G7G3/1 | Dako | M3575 | 1: 100 |
Others | |||||
CK20 | IMF | Ks 20.8 | Dako | M7019 | 1: 200 |
CD10 | neutral endopeptidase 24.11 | SS2/36 | Dako | M0727 | 1: 50 |
CD34 | single-chain transmembrane protein | QBEnd 10 | Dako | M7165 | 1: 100 |
CD68 | glycosylated lysosomal membrane protein | KP1 | Dako | M814 | 1: 3,000 |
CD99 | MIC2 gene products | 12E7 | Dako | M3601 | 1: 100 |
Bcl-2 | apoptosis inhibitor | 124 | Dako | M0887 | 1: 200 |
SMA | microfilaments | 1A4 | Dako | M0851 | 1: 300 |
Desmin | IMF | D33 | Dako | M0760 | 1: 100 |
S-100 | Calcium-binding proteins | polyclonal | Dako | Z0311 | 1: 3,000 |
HMB-45 | part of neuraminidase-sensitive glycoconjugate in melanosomes | HMB45 | Dako | M0634 | 1: 100 |
HHF-35 | muscle actin | HHF 35 | Dako | M0635 | 1: 200 |
MIB1 | nuclear protein, proliferation marker | Ki-67/MIB-1 | Dako | M7240 | 1: 200 |