|
GFAP (Dako, Copenhagen, Denmark)
|
No pre-treatment
|
120 min, 20°C
|
1:300
|
|
TNFα (Santa Cruz, CA, USA)
|
boiling in 0.1 M Citric Acid buffer.
|
120 min, 20°C
|
1:600
|
|
IL-1β (Santa Cruz, CA, USA)
|
boiling in 0.25 mM EDTA buffer.
|
120 min, 20°C
|
1:4000
|
|
IL-6 (Santa Cruz, CA, USA)
|
5 min Proteolytic Enzyme (Dako, Copenhagen, Denmark), 20°C.
|
120 min, 20°C
|
1:2000
|
|
CD68 (Serotec, United Kingdom)
|
5 min Proteolytic Enzyme (Dako, Copenhagen, Denmark), 20°C.
|
120 min, 20°C
|
1:200
|
|
HSP 27 (NovaCastra, United Kingdom)
|
boiling in 0.25 mM EDTA buffer..
|
120 min, 20°C
|
1:20
|
|
HSP 70 (NovaCastra, United Kingdom)
|
boiling in 0.25 mM EDTA buffer..
|
Overnight, 20°C
|
1:100
|
|
HSP 90 (NovaCastra, United Kingdom)
|
boiling in 0.25 mM EDTA buffer..
|
120 min, 20°C
|
1:50
|
|
COX2 (Santa Cruz, CA, USA)
|
5 min Proteolytic Enzyme (Dako, Copenhagen, Denmark), 20°C.
|
120 min, 20°C
|
1:100
|
|
ORP-150 (IBL, Gunma, Japan)
|
boiling in 0.1 M Citric Acid buffer.
|
120 min, 20°C
|
1:200
|
|
β-APP (NovaCastra, United Kingdom)
|
boiling in 0.1 M Citric Acid buffer.
|
Overnight, 20°C
|
1:300
|
|
TrypH (NovaCastra, United Kingdom)
|
boiling in 0.1 M Citric Acid buffer.
|
Overnight, 20°C
|
1:50
|
|
GAP-43 (Santa Cruz, CA, USA)
|
boiling in 0.1 M Citric Acid buffer
|
120 min, 20°C
|
1:2000
|
|
TdT enzyme (Chemicon, CA, USA)
|
15 min in Proteinase K (20 μg/ml), 20°C
|
60 min, 38°C
|
30 μl of TdT in 70 μl of reaction buffer (Chemicon, CA, USA)
|
