Table 1 Souce, dilution, pretreatment and cutoff values of primary antibodies used
From: Maspin expression is frequent and correlates with basal markers in triple-negative breast cancer
Antibody (clone) | Manufacturer | Dilution | Pretreatment | Cutoff values |
|---|---|---|---|---|
Maspin (EAW24) | Novocastra | 1:200 | Microwave | ≧10% (positive) |
CK5/6 (D5/16B4) | Dako | 1:50 | Water bath | ≧10% (positive) |
CK14 (LL002) | Novocastra | 1:100 | Water bath | ≧10% (positive) |
EGFR (EGFR.113) | Novocastra | 1:25 | Microwave | ≧10% (positive) |
CD10 (56C6) | Novocastra | 1:50 | Water bath | ≧10% (positive) |
p63 (4A4+Y4A3) | LabVision | 1:200 | None | ≧10% (positive) |
ER (1D5) | Dako | 1:50 | Water bath | ≧10% (positive) |
PgR (PgR636) | Dako | 1:800 | Water bath | ≧10% (positive) |
HER2 | Dako | Prediluted (Hercep test) | Water bath | Score 3+ (positive) |