Table 1 Antibodies and immunohistochemical procedures
Antibody | Source | Type | Clone | Positive control | Dilution | Incubation temperature | Incubation time |
|---|---|---|---|---|---|---|---|
Ki-67/MIB-1 | Dako, Glostrup, DK | Monoclonal | Ki-67 antigen | Tonsil | 1:100 | Room temperature | 40 min |
PHH3 | Upstate Biotechnology. Millipore, Billerica, USA | Polyclonal | H3 (Ser10) | Tonsil | 1:2000 | Room temperature | 60 min |
Survivin | Abcam Products, Cambridge, MA, USA | Monoclonal | EP2880Y | Tonsil | 1:250 | Room temperature | 40 min |
Mitosin | Novus Biologicals, LTD, Cambridge, UK | Monoclonal | 14C10/1D8 | Tonsil | 1:500 | Room temperature | 40 min |
DNA topoisomerase IIα | Dako, Glostrup, DK | Monoclonal | KiS1 | Tonsil | 1:100 | Room temperature | 40 min |