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Table 1 Antibodies and immunohistochemical procedures

From: Evaluation of the proliferation markers Ki-67/MIB-1, mitosin, survivin, pHH3, and DNA topoisomerase IIα in human anaplastic astrocytomas - an immunohistochemical study

Antibody Source Type Clone Positive control Dilution Incubation temperature Incubation time
Ki-67/MIB-1 Dako, Glostrup, DK Monoclonal Ki-67 antigen Tonsil 1:100 Room temperature 40 min
PHH3 Upstate Biotechnology. Millipore, Billerica, USA Polyclonal H3 (Ser10) Tonsil 1:2000 Room temperature 60 min
Survivin Abcam Products, Cambridge, MA, USA Monoclonal EP2880Y Tonsil 1:250 Room temperature 40 min
Mitosin Novus Biologicals, LTD, Cambridge, UK Monoclonal 14C10/1D8 Tonsil 1:500 Room temperature 40 min
DNA topoisomerase IIα Dako, Glostrup, DK Monoclonal KiS1 Tonsil 1:100 Room temperature 40 min