Table 2 Quantitative methylation-specific PCR primer and TaqMan probe sequences used in the present study
Genes | Forward primer sequence (5'→3') | Probe sequence (5'→3') | Reverse primer sequence (5'→3') |
|---|---|---|---|
CALCA | GTTTTGGAAGTATGAGGGTGACG | 6FAM-ATTCCGCCAATACACAACAACCAATAAACG-TAMRA | TTCCCGCCGCTATAAATCG |
CDH1 | AATTTTAGGTTAGAGGGTTATCGCGT | 6FAM-CGCCCACCCGACCTCGCAT-TAMRA | TCCCCAAAACGAAACTAACGAC |
DAPK1 | GGATAGTCGGATCGAGTTAACGTC | 6FAM-TTCGGTAATTCGTAGCGGTAGGGTTTGG-TAMRA | CCCTCCCAAACGCCGA |
IRX2 | GCGGGTCGTTTAGGTTAGTATTCG | 6FAM-CCCTCCATCCACGCCCGACCGAAA-TAMRA | CGCCGAACAACGAACAAAACG |
TIMP3 | GCGTCGGAGGTTAAGGTTGTT | 6FAM-AACTCGCTCGCCCGCCGAA-TAMRA | CTCTCCAAAATTACCGTACGCG |
PAX6 | ATATAGGACGGCGGTTTAGGTTG | 6FAM-CCCAAAATCCGACCGACTCCAACCCCTA-TAMRA | TTCCGACCGAACGAAAACCTAC |
β-Actin | TGGTGATGGAGGAGGTTTAGTAAGT | 6FAM-ACCACCACCCAACACACAATAACAAACACA-TAMRA | AACCAATAAAACCTACTCCTCCCTTAA |