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Table 1 List of PCR primers used for EBV subtyping and detection of LMP1 gene deletion

From: Aberrant antigenic expression in extranodal NK/T-cell lymphoma: a multi-parameter study from Thailand

Type of Primer DNA sequence Product size
EBNA2 EBV type A 2A-5' 5'-AACTTCAACCCACACCATCA-3' 116 bp
  EBV type B 2A-3' 5'-TTCTGGACTATCTGGATCAT-3' 120 bp
EBV LMP-1 wild-type 1-A 5'-CGACTCTGCTGGAAATGATGGAGGC-3' 210 bp
  deletion-type 1-B 5'-TGAACTGGGCCGTGGGGGTCGTCAT-3' 180 bp
β-actin (semi-nested PCR) 1st forward SFW 5'-CAAGAGATGGCCACGGCTGCT-3'  
  2nd forward S4FW 5'-GAGGCACTCTTCCAGCCTTCC-3' 148 bp
  reverse A5RV 5'-TCGTGGATGCCACAGGACTCC-3'  
  1. EBNA2, Epstein-Barr virus nuclear antigen 2 gene; LMP1, latent membrane protein 1 gene