Table 1 List of PCR primers used for EBV subtyping and detection of LMP1 gene deletion

EBNA2

EBV type A

2A-5'

5'-AACTTCAACCCACACCATCA-3'

116 bp

EBV type B

2A-3'

5'-TTCTGGACTATCTGGATCAT-3'

120 bp

EBV LMP-1

wild-type

1-A

5'-CGACTCTGCTGGAAATGATGGAGGC-3'

210 bp

deletion-type

1-B

5'-TGAACTGGGCCGTGGGGGTCGTCAT-3'

180 bp

β-actin (semi-nested PCR)

1^st forward

SFW

5'-CAAGAGATGGCCACGGCTGCT-3'

2^nd forward

S4FW

5'-GAGGCACTCTTCCAGCCTTCC-3'

148 bp

reverse

A5RV

5'-TCGTGGATGCCACAGGACTCC-3'