Table 1 List of antibodies and staining results.
Antibody | Company | Dilution | Tumor cells |
|---|---|---|---|
Vimentin | DAKO | 1:1000 | positive |
HLA-DR | DAKO | 1:200 | part.positive |
Fascin | Abcam | 1:100 | positive |
Langerin | eBioscience | 1:50 | negative |
Lysozym | DAKO | 1:400 | negative |
S100 | DAKO | 1:5000 | negative |
AE1/3 | DAKO | 1:100 | negative |
HMB45 | DAKO | 1:50 | negative |
MITF | Zytomed | 1:100 | negative |
PMZ | Zytomed | 1:50 | negative |
LCA | DAKO | 1:500 | negative |
Calponin | DAKO | 1:200 | negative |
Caldesmon | DAKO | 1:50 | negative |
Clusterin | Menarini | 1:30 | negative |
EMA | DAKO | 1:100 | negative |
SMA | DAKO | 1:500 | negative |
CK5/6 | DAKO | 1:100 | negative |
CK8/18 | Zytomed | 1:100 | negative |
OSCAR | Zytomed | 1:200 | negative |
Synaptophysin | DCS | 1:200 | negative |
Chromogranin | DAKO | 1:500 | negative |
Factor XIIIa | DCS | 1:100 | negative |
CD1a | DAKO | 1:50 | negative |
CD3 | Santa Cruz | 1:200 | negative |
CD4 | Zytomed | 1:200 | negative |
CD20 | DAKO | 1:500 | negative |
CD21 | Zytomed | 1:50 | negative |
CD23 | Novocastra | 1:30 | negative |
CD30 | DAKO | 1:50 | negative |
CD31 | DAKO | 1:100 | negative |
CD34 | DAKO | 1:50 | negative |
CD35 | Novocastra | 1:50 | negative |
CD56 | Cell Marque | 1:100 | negative |
CD68 (PG-M1) | DAKO | 1:200 | negative |
CD117 | DAKO | 1:200 | negative |
CD123 | BD Pharmingen | 1:50 | negative |
Mib1 | DAKO | 1:200 | 40-50% |