Figure 2

Pathological examinations. a: Mineralized bone tissues are colored in green and nonmineralized osteoid tissues are shown in orange by Villanueva-Goldner stain. Mineralized bone tissues are colored in purple and nonmineralized osteoid tissues are shown in the clear zone by Villanueva bone stain. The osteoids volume/mineralized bone volume ratio was 20.7% (normal range, less than 10%). Osteoid thickness was 25.1 μm (average, less than 12.5 μm). b: Tetracycline labeling examination showed no double-labeling pattern. These findings indicate mineralization deficiency and osteomalacia.