Microscopic views and T-cell receptor rearrangement of the tumors. HE (A) and immunohistochemistry against CD3 (B), CD20 (C), CD56 (D), TIA-1 (E) and Ki-67 (F). (G) In situ hybridization against EBER-1. (H) Southern blotting to detect T-cell receptor rearrangement in fresh frozen tumor tissue. The first column in each blot indicates the size of the DNA fragment (kbp). Restriction enzymes used were as the follows: E, EcoRI; B, BamHI and H, HindIII.