Figure 2

Immunohistochemical staining. A: The liver tissues surrounding the tumor were diffusely positive staining for AE1/AE3. B: The histiocytoid cells were faintly positive staining for AE1/AE3. C-E: The histiocytoid cells were strongly diffuse positive staining for vimentin, HMB-45, melan-A. F: The histiocytoid cells were focally positive staining for smooth muscle actin, especially the cells around blood vessel. G: Scattered tumor cells were positive for CD68. H-J: The histiocytoid cells were negative staining for CD21, S100 and ALK. K and L: The lymphocytes among the histiocytoid cells were mainly positive for CD3 and focally positive for CD20, whereas the histiocytoid cells were negative. M and N: CD31 and CD34 underlined the rich vascular channels, whereas the histiocytoid cells were negative. O: Ki67 index was about 5%.