Immuno-electron-microscopy. Immuno-electron-microscopic detection of pHp, SP-B and SP-A (LB: lamellar body, N: nucleus). Image-acquisition was performed using a Zeiss EM 910 transmission-electron-microscope with built in analogue camera (Carl Zeiss, Germany). Negatives were scanned with a Flextight X5 scanner with FlexColor software version 4.8.8. (Hasselblad, Sweden). A: pHp in a lamellar body (5000x). B: SP-B in a lamellar body (5000x). C: Surfactant protein-A in a not lamellar vesicle (5000x). D: Negative control (5000x). Two independent experiments.