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Table 2 Antigens used for generation of polyclonal antibodies

From: Immunohistochemical analysis of medullary breast carcinoma autoantigens in different histological types of breast carcinomas

Antigen Full name NCBI reference DNA fragment, bp Vector MW of recombinant protein, kDa, (including tag)
LGALS3BP Homo sapiens lectin, galactoside-binding, soluble, 3 binding protein NM_005567.2 1483-1686 pGEX4T3 (GST-tag) 35
RAD50 Homo sapiens RAD50 homolog (S. cerevisiae) NM_005732.2 2552-3374 pET28b (6His-tag) 44
FAM50A Homo sapiens family with sequence similarity 50, member A NM_004699.1 76-1095 pGEX4T3 (GST-tag) 64
RBPJ Homo sapiens recombination signal binding protein for immunoglobulin kappa J region NM_203284.1 492-1850 pET28b (6His-tag) 50
PABCP4 Homo sapiens poly(A) binding protein, cytoplasmic 4 (inducible form) NM_003819.2 927-3052 pET28b (6His-tag) 67
LRRFIP1 Homo sapiens leucine rich repeat (in FLII) interacting protein 1 NM_004735.2 417-1804 pET28b (6His-tag) 51