Table 2 Antigens used for generation of polyclonal antibodies
Antigen | Full name | NCBI reference | DNA fragment, bp | Vector | MW of recombinant protein, kDa, (including tag) |
|---|---|---|---|---|---|
LGALS3BP | Homo sapiens lectin, galactoside-binding, soluble, 3 binding protein | NM_005567.2 | 1483-1686 | pGEX4T3 (GST-tag) | 35 |
RAD50 | Homo sapiens RAD50 homolog (S. cerevisiae) | NM_005732.2 | 2552-3374 | pET28b (6His-tag) | 44 |
FAM50A | Homo sapiens family with sequence similarity 50, member A | NM_004699.1 | 76-1095 | pGEX4T3 (GST-tag) | 64 |
RBPJ | Homo sapiens recombination signal binding protein for immunoglobulin kappa J region | NM_203284.1 | 492-1850 | pET28b (6His-tag) | 50 |
PABCP4 | Homo sapiens poly(A) binding protein, cytoplasmic 4 (inducible form) | NM_003819.2 | 927-3052 | pET28b (6His-tag) | 67 |
LRRFIP1 | Homo sapiens leucine rich repeat (in FLII) interacting protein 1 | NM_004735.2 | 417-1804 | pET28b (6His-tag) | 51 |