Table 1 Panel of immunohistochemical stains
Immunohistochemical stains | Clone | Sources | Dilution | Results |
|---|---|---|---|---|
Pan cytokeratin | AE1/AE3 | Neomarkers | 1:100 | Neg. |
Keratin 8/18 | 5D3 | Neomarkers | 1:200 | Neg. |
Keratin 19 | RCK108 | Neomarkers | 1:200 | Neg. |
Renal Cell Carcinoma Marker(gp200) | PN-15 | Neomarkers | 1:100 | Neg. |
Neuron-specific enolase (NSE) | NSE-P1 | Neomarkers | 1:1500 | Pos. |
Epithelial membrane antigen (EMA) | E29 | Neomarkers | 1:500 | Pos. (focal) |
CD10 | 56 C6 | Neomarkers | 1:50 | Pos. (focal) |
Ki-67 | SP6 | Neomarkers | 1:500 | Pos. (1%) |
Vimentin | SP20 | Neomarkers | 1:300 | Pos. |
S100 protein | Polyclonal antiserum | Dako | 1:6000 | Pos. |
a-inhibin | R1 | Dako | 1:1250 | Pos. |
Epidermal growth factor receptor (EGFR) | EP38Y | Neomarkers | 1:100 | Pos. |
calretinin | SP13 | Neomarkers | 1:100 | Neg. |
Melanoma Marker | HMB45 | Neomarkers | 1:50 | Neg. |
Melan-A | A103 | Neomarkers | 1:100 | Neg. |
Chromogranin A | SP12 | Neomarkers | 1:200 | Neg. |
Desmin | Rabbit Polyclonal Antibody | Neomarkers | 1:200 | Neg. |
Actin | HHF35 | Neomarkers | 1:100 | Neg. |
Myoglobin | Rabbit Polyclonal Antibody | Neomarkers | 1:800 | Neg. |
CD68 | PG-M1 | Neomarkers | 1:100 | Neg. |
CD34 | QBEnd/10 | Neomarkers | 1:80 | Neg. |