Manual and automated annotations of ovarian serous carcinoma. Ovarian serous carcinoma TMA spots immunohistochemically stained for S100A1. Representative lowly and highly stained spots are shown (A-B). Image data were processed by both manual pathologist-supervised hand annotations and automated Genie Histology Pattern Recognition software. Digital hand annotations are presented as green outlines of carcinoma, excluding stroma and minimizing background and glass (C-D). These same TMA spots were classified by Genie as carcinoma (dark blue), stroma (yellow), and glass (light blue) (E-F).