Representative comparisons of pathologist visual scoring with automated IHC measurement. Ovarian serous carcinoma TMA spots stained for S100A1 were interpreted by pathologist visual scoring as 0 (no staining), 1 (<10% of carcinoma staining), 2 (10%-50% of carcinoma staining), or 3 (>50% of carcinoma staining). Representative spot for each score is shown as A-D; each column shows the identical TMA spot processed by digital methods. Genie Histology Pattern Recognition software classified tissue areas into carcinoma (dark blue), stroma (yellow), or glass (light blue) (E-H). Color Deconvolution software individually analyzed DAB staining (deconvolved by its RGB color components; I-L), and measured staining intensity only within areas classified as carcinoma. Pseudocolors represent staining intensity in shown as M-P (gray = image areas not annotated by Genie as carcinoma and therefore not considered; blue = no staining, yellow = low intensities, orange = medium intensities, and red = high intensities in Genie-annotated carcinomatous areas considered).