Figure 4From: Quantitative comparison of immunohistochemical staining measured by digital image analysis versus pathologist visual scoringRepresentative comparisons of pathologist visual scoring with automated IHC measurement. Ovarian serous carcinoma TMA spots stained for S100A1 were interpreted by pathologist visual scoring as 0 (no staining), 1 (<10% of carcinoma staining), 2 (10%-50% of carcinoma staining), or 3 (>50% of carcinoma staining). Representative spot for each score is shown as A-D; each column shows the identical TMA spot processed by digital methods. Genie Histology Pattern Recognition software classified tissue areas into carcinoma (dark blue), stroma (yellow), or glass (light blue) (E-H). Color Deconvolution software individually analyzed DAB staining (deconvolved by its RGB color components; I-L), and measured staining intensity only within areas classified as carcinoma. Pseudocolors represent staining intensity in shown as M-P (gray = image areas not annotated by Genie as carcinoma and therefore not considered; blue = no staining, yellow = low intensities, orange = medium intensities, and red = high intensities in Genie-annotated carcinomatous areas considered).Back to article page