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Figure 2 | Diagnostic Pathology

Figure 2

From: Expression features of SOX9 associate with tumor progression and poor prognosis of hepatocellular carcinoma

Figure 2

Increased SOX9 protein and mRNA levels in hepatocellular carcinoma (HCC) with different TNM stages and adjacent nonneoplastic liver tissues. (a) Representative Western blotting of SOX9 protein levels in HCC tissues and adjacent nonneoplastic liver tissues. (b) Semiquantitative Western blotting showed that the expression levels of SOX9 protein were significantly higher than those in adjacent nonneoplastic liver tissues (P  0.01). Additionally, the expression levels of SOX9 protein were increased with ascending tumor TNM stages. GAPDH was used as internal control. Means, standard deviation (SD), and P values were given (T test). (c) Quantitative RT-PCR assay showed significantly increased SOX9 mRNA level in HCC tissues compared with adjacent nonneoplastic liver tissues (P  0.01). Additionally, the expression levels of SOX9 mRNA were increased with ascending tumor TNM stages. GAPDH was used as internal control. Means, standard deviation (SD), and P values were given (Mann–Whitney test).

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