Figure 3

Gene arrangement assays of BCR (IgH, IgK, IgL) and TCR in the tumor tissues. Positive gene arrangements of BCR (IgH, IgK and IgL) could be detected in the tumor tissues, but TCR β, δ and γ could not be. a. BCR (IgH, IgK, IgL) gene arrangement assays. Lane 1. IgH: VH-FR1 + JH consensus (+); Lane 2, IgH: VH-FR2+ JH consensus (+); Lane 3, IgH: VH-FR3+ JH consensus (+); Lane 4, DH + JH consensus (−); Lane 5, DH7+ consensus (+); Lane 6, IgK: Vκ + Jκ (−); Lane 7, IgK: Vκ and intron + Kde (+); Lane 8, IgL: Vκ + Jλ (+); Lane 9, Marker. b. TCR gene arrangement assays. Lane 1, DNA ladder; Lane 2, TCRβ: Vβ + Jβ1/2 (−); Lane 3, TCRβ: Vβ + Jβ2 (−); Lane 4, TCRβ: Dβ + Jβ1/2 (−); Lane 5, TCRδ: Vδ + Dδ + Jδ (−); Lane 6. TCRγ: Vγ1-8, Vγ10 + multiple Jγ regions (−); Lane 7, TCRγ: Vγ9, Vγ11 + multiple Jγ regions (−); Lane 8, Marker.