Table 2 Targeted Mutations and Pyrosequencing dispensing order for EGFR, KRAS and BRAF mutations
From: Pyrosequencing data analysis software: a useful tool for EGFR, KRAS, and BRAF mutation analysis
Targeted Mutations | Pyrosequencing Dispensing Orders |
|---|---|
EGFR exon 18, codon 719: | CATGTCACTCGTG |
EGFR exon 19 deletions | CTATCACTGTCAGCTCGATCGTCATCGTCACGC |
EGFR exon 20, Codon 768 and insertions | GCAGTACGTGTCGTGTACGTGACCACACTG |
EGFR exon 20, Codon 790 | GATTCATCTG |
EGFR exon 21 | ACGTGTCACATGTC |
KRAS codon 12 & 13 | ACTGTACGTGATCGTAGCAAGAG |
KRAS codon 61 (reverse sequencing) | GCTCGATACGACCT |
BRAF codon 600 (reverse sequencing) | TCGTATCTGTAG |