Table 7 Gene-protein IHC/BISH (comparator) vs. single IHC/BISH (reference) agreement analyses
Single IHC/BISH assay | ||||
|---|---|---|---|---|
| Â | Gene-protein IHC/BISH | Positive | Negative | Total |
Reader A | Positive | 50 | 2 | 52 |
| Â | Negative | 1 | 112 | 113 |
| Â | Total | 51 | 114 | 165 |
|  | PPA: n/N (%) (95% CI) | 50/51 (98.0) (89.7–99.7) |  |  |
|  | NPA: n/N (%) (95% CI) | 112/114 (98.2) (93.8–99.5) |  |  |
|  | OPA: n/N (%) (95% CI) | 162/165 (98.2) (94.8–99.4) |  |  |
|  | Kappa coefficient (95% CI) | 0.96 (0.91–1.00) |  |  |
Reader B | Positive | 50 | 2 | 52 |
| Â | Negative | 3 | 117 | 120 |
| Â | Total | 53 | 119 | 172 |
|  | PPA: n/N (%) (95% CI) | 50/53 (94.3) (84.6–98.1) |  |  |
|  | NPA: n/N (%) (95% CI) | 117/119 (98.3) (94.1–99.5) |  |  |
|  | OPA: n/N (%) (95% CI) | 167/172 (97.1) (93.4–98.8) |  |  |
|  | Kappa coefficient (95% CI) | 0.93 (0.87–0.99) |  |  |
Reader C | Positive | 52 | 3 | 55 |
| Â | Negative | 2 | 116 | 118 |
| Â | Total | 54 | 119 | 173 |
|  | PPA: n/N (%) (95% CI) | 52/54 (96.3) (87.5–99.0) |  |  |
|  | NPA: n/N (%) (95% CI) | 116/119 (97.5) (92.8–99.1) |  |  |
|  | OPA: n/N (%) (95% CI) | 168/173 (97.1) (93.4–98.8) |  |  |
|  | Kappa coefficient (95% CI) | 0.93 (0.88–0.99) |  |  |