Table 1 Nucleotide primer sequences used for real-time quantitative PCR
Target genes | Nucleotide sequence | Orientation | Amplicon length | Accession No. |
|---|---|---|---|---|
Bak | 5′— CCCAGGACACAGAGGAGGTTT—3′ 5′— GCCTCCTGTTCCTGCTGATG—3′ | Sense Antisense | 65 bp | NM001188 |
Bcl-xl | 5′— TGCGTGGAAAGCGTAGACAA—3′ 5′—ATTCAGGTAAGTGGCCATCCAA—3′ | Sense Antisense | 75 bp | NM138578 |
Mcl-1 | 5′— AGGCTGGGATGGGTTTGTG—3′ 5′—CACATTCCTGATGCCACCTTCT—3′ | Sense Antisense | 65 bp | NM021960 |
GAPDH | 5′- AACAGCCTCAAGATCATCAGCA-3′ 5′- CATGAGTCCTTCCACGATACCA-3′ | Sense Antisense | 102 bp | M33197 |