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Figure 4 | Diagnostic Pathology

Figure 4

From: Analysis of aberrant methylation on promoter sequences of tumor suppressor genes and total DNA in sputum samples: a promising tool for early detection of COPD and lung cancer in smokers

Figure 4

Representative examples of MS-PCR analysis of 82 induced sputum clinical samples. Bisulfite-modified DNA was amplified with specific primers to detect methylated DNA (M) and unmethylated DNA (U); MW: Molecular weight marker; in vitro genomic DNA treated with SssI enzyme used as positive control for methylated genes. (CM); peripheral blood lymphocytes DNA used as a control of unmethylated genes (CU); H20: distilled water used as negative control in the PCR amplification.

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Diagnostic Pathology

ISSN: 1746-1596

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