The expression pattern of CARM1 in human invasive breast cancer (×400). A In positive samples, the expression of CARM1 in adenocarcinomas was considerably increased compared with the adjacent benign glandular epithelium ( A and B). The increased expression was both nuclear and cytoplasmic (C), predominantly nuclear (D), or predominantly cytoplasmic (E). A representative negative case is shown (F). Asterisks indicate adenocarcinoma. Arrows indicate benign breast epithelium. B CARM1 expression was increased significantly in invasive breast cancer compared with benign epithelium (one-way ANOVA P < 0.001).