Figure 4

Genetic features of inflammatory myofibroblastic tumor. (A) Reverse-transcription polymerase chain reaction (RT-PCR) with RANBP2 and ALK primers. Lane T1: no amplification band was observed in Case 1. Lane T2: an expected 254-bp product was present in Case 2. Lane C: tonsil tissue was adopted as a control, and no amplification band was observed. Lane NTC: no template control. (B) Detection of house-keeping gene of beta-actin by RT-PCR. An expected 285-bp product was seen in Case 2 (Lane 2) and tonsil tissue (Lane 3). No amplification band was observed in Case 1 (Lane 1) and no-template control (Lane NTC). (C) The fusion point of the RANBP2-ALK gene as indicated by cDNA sequencing was located between exon 18 of the RANBP2 gene and exon 20 of the ALK gene.