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Table 2 Antibodies used for immunohistochemistry

From: Increased expression of α-methylacyl-coenzyme A racemase (AMACR; p504s) and p16 in distal hyperplastic polyps

Antibody

 

Pretreatment

Incubation

Detection

Dilution

Company

AMACR

Monoclonal

98°C Hot water, pH 9.0,

15 min

Envision™

Ready-to-Use Kit

Dako, Glostrup, Denmark

p16Ink

Monoclonal, clone G175-405

98°C Hot water, pH 6.1,

20 min

Envision™

1:10

BD Biosciences, Heidelberg, Germany

Ki67

Monclonal, clone MIB-1

98°C Hot water, pH 9.0,

15 min

Envision™

Ready-to-Use Kit

Dako, Glostrup, Denmark

BRAF

Monoclonal, clone VE1

37°C Hot water, PH 9.0

30 min

Envision™

1:100

DCS Innovative Diagnostik, Hamburg, Germany

MLH1

Monoclonal, clone ES05

98°C, pH 9.0

20 min

Envision™

Ready-to-Use Kit

Dako, Glostrup, Denmark

Beta-catenin

Monoclonal

37°C water bath 30 min

20 min

Zytochem Plus HRP Polymer (Zytomed Systems, Berlin, Germany)

1:2000

BD Biosciences, Heidelberg, Germany

CK20

Monoclonal, Clone Ks20.8

98°C, pH 9.0

30 min

Envision™

Ready-to-Use Kit

Dako, Glostrup, Denmark