Immunostains of tissue biopsy. (A): 10× H&E; the dermis was infiltrated by sheets and nests of tumor cells with eosinophilic cytoplasm and large nuclei with prominent nucleoli, the epidermis was uninvolved. (B): 20× H&E; higher power of tumor within subcutis shows a nested tumor architecture, neoplastic cells are somewhat discohesive and have bright eosinophilic cytoplasm. (C): 20× S-100; positive nuclear and cytoplasmic reactivity in tumor cells for S-100. (D): 20× HMB-45; positive cytoplasmic reactivity. (E): 20× Melan-A; positive cytoplasmic reactivity. (F): 20× MITF; positive nuclear reactivity.