Table 1 List of all primers used and conditions of PCR amplification
From: Hypermethylation of EDNRB promoter contributes to the risk of colorectal cancer
Primer | Sequence (5′-3′) | Product size (bp) | |
|---|---|---|---|
M | F | CGAAGAGGTTGCGGGCGGTATTAGCG | 130 |
R | TACTCCAAAAACGTCCGATAACCG | ||
U | F | TGGTGAAGAGGTTGTGGGTGGTATTAGTG | 134 |
R | ACCTACTCCAAAAACATCCAATAACCA | ||
RT-PCR | |||
EDNRB-F | GAAAGCCTCCGTGGGAATC | 86 | |
EDNRB-R | ACAGCTCGATATCTGTCAATACTCAGA | ||
GAPDH-F | CCATGGAGAAGGCTGGGG | 194 | |
GAPDH-R | CAAAGTTGTCATGGATGACC |