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Table 2 Sequences of oligodeoxyribonucleotides

From: Immunohistochemical detection of mutations in the epidermal growth factor receptor gene in lung adenocarcinomas using mutation-specific antibodies

Name

Sequence (5′→3′)1

Position

19 F

GCCAGTTAACGTCTTCCTTCTCTC

Intron 18

19R

YGAGCAGGGTCTAGAGCAGAGCA

Intron 19

21 F

CGGATGCAGAGCTTCTTCCCATG

Intron 20

21R

CTAGTGGGAAGGCAGCCTGGTC

Intron 21

CLMP1

+T+T+A+A+GA+GA+A+G+C+AA+C+A+T+CT-C3

Exon 19

CLMP2

+T+G+G+G+C+T+GGCA-C3

Exon 21

  1. 1Other than the bases A, T, C and G, the oligos are described by a Y for mixed T and C; the positions of the LNA nucleotides (+ before the LNA) in the clamps (CLMP1 and CLMP2); and the C3 spacer (-C3) on the 3′ terminus of the clamps.