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Table 3 Intra- and inter-observer agreement based on slides treated with different antigen retrieval buffers

From: Immunohistochemical detection of mutations in the epidermal growth factor receptor gene in lung adenocarcinomas using mutation-specific antibodies

Antigen retrieval buffer Inter-observer agreement Fleiss’ Kappa Intra-observer agreement Cohen’s Kappa
  Value 95% CI Value 95% CI
EDTA (pH 8.0) 0.912 (0.862, 0.912) 0.955 (0.918, 0.992)
Na Citrate (pH 6.0) 0.753 (0.677, 0.829) 0.853 (0.790, 0.916)
EDTA (pH 9.0) 0.643 (0.558, 0.728) 0.801 (0.730, 0.872)
P < 0.05   < 0.05