17- DMAG decreases expression of IKKβ. Cells were treated with DMSO, TNF-α (10 ng/ml), 17-DMAG (0.05 μM), or both of TNF-α (10 ng/ml) and 17-DMAG for 40 hours. Whole-cell extracts were prepared and immunoblot analysis was performed to analyze the expression of IKKβ, β-actin. The cellular β-actin served as a loading control. (A) NuLi-1 cells; (B) MS-1 cells; (C) A549 cells; (D) LK-2 cells.