Figure 2

Immunohistochemical staining for E-cadherin, β-catenin, and S100A4 in colorectal cancers (×400). E-cadherin, membranous staining at the center of the tumor (A, arrow) and loss or attenuation of expression at the invasive margin of tumor buds (B, arrow). β-catenin, membranous staining (C, arrow) and nuclear staining at the invasive margin of tumor buds (D, arrow). Negative (E, arrow) and positive (F, arrow) staining for S100A4.