Figure 1

Digital analysis of scanned slides The DAB-imunostaining levels are detected on the brown-channel. MembraneQuant detects the nuclei on the blue channel to identify the cells of the ROI, than calculates an intensity topographic map to classify each cell to a distinct class, which is later on calculated to a field score (here the strong, complete membrane staining was of a 3+ score). On the result-slide the positivity-class of the cells are color-coded (blue: nucleus, yellow: 1+, orange: 2+, red: 3+).