Is HER2 amplification predictable by digital immunohistochemistry?

Diagnostic Pathology

Table 1 Differences in membrane-stainings according to FISH-positivity.

	FISH -	FISH +	FISH -	FISH +	FISH -	FISH +	FISH -	FISH +	FISH -	FISH +	FISH -	FISH +
p=	0.07097		0.01782		0.05574		0.00031		0.00166		0.00486
	HER2 2+ % RATIO	HER2 2+ % RATIO	HER2 3+ % RATIO	HER2 3+ % RATIO	H-Score	H-Score	L0_CMA:1+	L0_CMA:1+	L0_CMA:2+	L0_CMA:2+	L0_CMA:3+	L0_CMA:3+
Average	0.08386	0.152766	0.012607	0.04071	61.25344	88.80895	7315.882	25794.81	1317.471	12464.48	184.7127	2866.724
SD	0.107272	0.135455	0.024028	0.047413	42.95535	48.74765	4898.122	21455.78	1796.785	15429.72	365.2118	4210.592
Min.	0	0	0	0	3.007519	9.550562	207.6841	1472.817	0	0	0	0
Max.	0.28777	0.408517	0.098592	0.162461	136.6906	170.5047	19422.9	67658.91	5221.888	46501.73	1549.739	11813.45

MembraneQuant calculates intensity scores for each cell within an annotation and additional proportional score. Significant difference is highlighted underlining; italics indicates strong tendency. FISH+ cases had significantly higher HER2-IHC3+ proportional score, while IHER2-IHC2+ proportional score and H-score had a tendency of higher occurrence in the FISH+ cases. In our set, the most significant differences (underlined) were found between FISH-positive and negative cases in the label-area of different IHC-intensity classes.

ISSN: 1746-1596