Table 1 Primer sequences for KIT, PDGR, EGFR, KRAS PCR amplification
From: KRAS, EGFR, PDGFR-α, KIT and COX-2 status in carcinoma showing thymus-like elements (CASTLE)
KIT9F 5′GCC ACA TCC CAA GTG TTT TAT G | KIT9R 5′GAG CCT AAA CAT CCC CTT AAA TTG |
|---|---|
KIT11F 5′CCA GAG TGC TCT AAT GAC TG | KIT11R 5′AGC CCC TGT TTC ATA CTG AC |
KIT13F 5′CTT GAC ATC AGT TTG CCA GTT GT | KIT13R 5′GAC AGA CAA TAA AAG GCA GCT TG |
KIT14F 5′CTC ACC TTC TTT CTA ACC TTT TCT T | KIT14R 5′CCC ATG AAC TGC CTG TCA AC |
KIT17F 5′ATG GTT TTC TTT TCT CCT CC | KIT17R 5′TAC ATT ATG AAA GTC ACA GG |
PDGFRA10F 5′GGC CCT ATA CTT AGG CCC TTT T | PDGFRA10R 5′TGT CCT GAC TGT TGA GGA ACT |
PDGFRA12F 5′CTC TGG TGC ACT GGG ACT TT | PDGFRA12R 5′GCA AGG GAA AAG GGA GTC TT |
PDGFRA14F 5′TCT GAG AAC AGG AAG TTG GTA GC | PDGFRA14R 5′CCA GTG AAA ATC CTC ACT CCA |
PDGFRA18F 5′TCT TGC AGG GGT GAT GCT AT | PDGFRA18R 5′AGA AGC AAC ACC TGA CTT TAG AGA TTA |
EGFR18F 5′GCT GAG GTG ACC CTT GTC TC | EGFR18R 5′ACA GCT TGC AAG GAC TCT GG |
EGFR19F 5′GCT GGT AAC ATC CAC CCA GA | EGFR19R 5′GAG AAA AGG TGG GCC TGA G |
EGFR 20 F 5′CAT GTG CCC CTG CTT CTG | EGFR 20R 5′GAT CCT GGT TCC TTA TCT CC |
EGFR21F 5′CCT CAC AGC AGG GTC TTC TC | EGFR21R 5′CCT GGT GTC AGG AAA ATG CT |
KRAS2F 5′GTG TGA CAT GTT CTA ATA TAG TCA | KRAS2R 5′GAA TGG TCC TGC ACC AGT AA |
KRAS3F 5′CCA GAC TGT GTT TCT CCC TTC | KRAS3R 5′TGC ATG GCA TTA GCA AAG AC |