Figure 2

An adenocarcinoma ( case 1 ) with 4 different expression patterns and various combinations of heterogeneity, loss of MLH1 / PMS2 and heterogeneity / retained expression for MSH2 / MSH6. A) clonal loss of MLH1 staining. B) MSI corresponding to loss of staining, C) MSS corresponding to retained MMR protein staining. Methylation analysis revealed D) presence and E) absence, respectively, of MLH1 promoter methylation, which verifies clonal MLH1 methylation status. Flow cytometric analysis showing different DNA indices, i.e. F) 1.13 in the MSI area and G) 1.57 in the MSS area.